Protein Info for GFF421 in Xanthobacter sp. DMC5

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1242 PF08471: Ribonuc_red_2_N" amino acids 31 to 147 (117 residues), 151.8 bits, see alignment E=5.9e-49 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 132 to 749 (618 residues), 466.6 bits, see alignment E=6.6e-144 PF02867: Ribonuc_red_lgC" amino acids 198 to 749 (552 residues), 354.5 bits, see alignment E=1e-109 amino acids 830 to 912 (83 residues), 50.3 bits, see alignment 1.6e-17

Best Hits

Swiss-Prot: 77% identical to NRDJ_BRADU: Vitamin B12-dependent ribonucleotide reductase (nrdJ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 86% identity to azc:AZC_1268)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1242 amino acids)

>GFF421 hypothetical protein (Xanthobacter sp. DMC5)
MRVERRYTQEGRSPYADIAFRETVSEIRNPDGSVVFRQEGIEVPAQFSQVASDILAQKYF
RKAGVPARLKKVEESSVPSFLWRSVADHEALAGLPEKERLVGETSAKQVFDRLAGTWTYW
GWKGGYFDSEADAQAFFDEHCYMLAMQMAAPNSPQWFNTGLFWAYGIDGPGQGHYYVDYQ
TGELTASTSAYEHPQPHACFIQSVSDDLVSDGGIMDLWVREARLFKYGSGTGSNFSALRG
EGEKLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDADHPDIEAYVDWKVIE
EQKVSSLVTGSKLNAKHLKAVLKACVNCEGNGDDCFDPEKNPALKREIKAARKAAVADAA
IRRVIQYARQGYKDIDVAIYDTDWDGEAYITVAGQNSNNSVRVTDDFLRAVEADEDWKLI
RRTDGKVAKTIKARELWEKIGSAAWHCADPGIQFHTAVNDWHTCPAAGEIRASNPCSEYM
FLDDTACNLASLNLLQFRDAEKRFDVESYEHAVRLWTVVLEISVLMAQFPSRRIAELSYE
YRTLGLGYANIGGLLMSSGIPYDSPQGRAICGALSAIMTGVCYATSAEMAKELGTFAAYS
ANAQSMLRVIRNHRRAAHGIAEGYEGLSIAPVPLDHLSCPDTRLVAHATAAWDKALELGE
KHGYRNAQVSVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPEALRT
LGYKEAEIAEIEAYAVGHGSLANAPAVNHGSLRAKGFDDAAIQKAEAAVKTAFDIKFAFN
RWTFGEEFLANTLKVPAAQLADPKFDLLAFLGFSKADIDAANVHVCGAMTLEGAPFLKPQ
HLPVFDCASPCGRIGKRYLSVESHIRMMAAAQPFITGAISKTINMPNDASVEDCKAAYLL
SWRLALKANALYRDGSKLSQPLNAQLVADEEDEDDALDAVLAQPNAARTASVTEKIVERV
VERIVEHRVREREKMPDRRKGYTQKAVVGGHKVYLRTGEYDDGRLGEIFIDMHKEGAALR
SLLNNFAIAISLGLQYGVPLEEYVDAFTFTRFEPAGPVQGNDTIKYATSMLDYVFRELAV
SYLGRNDLGHVDLSETGFDALGKGVDEGKAPTNPSARIVSKGLVRGKTVNLLVPQPVAPE
PRSGTDNVTMLRTTTVGATALKADTAELKDPAPQPEEIEEAVESLMEKSLPWSATARKAE
ARAEAKAKGYEGEACPECMNFTMVRNGTCLKCDTCGGTTGCS