Protein Info for PS417_21530 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 transmembrane" amino acids 6 to 30 (25 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details amino acids 232 to 254 (23 residues), see Phobius details PF00672: HAMP" amino acids 253 to 304 (52 residues), 46.9 bits, see alignment 4.2e-16 PF00512: HisKA" amino acids 309 to 373 (65 residues), 51.4 bits, see alignment E=1.3e-17 PF02518: HATPase_c" amino acids 424 to 532 (109 residues), 85.1 bits, see alignment E=7e-28

Best Hits

KEGG orthology group: K07639, two-component system, OmpR family, sensor histidine kinase RstB [EC: 2.7.13.3] (inferred from 96% identity to pfs:PFLU4723)

Predicted SEED Role

"Sensor histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U5A9 at UniProt or InterPro

Protein Sequence (536 amino acids)

>PS417_21530 histidine kinase (Pseudomonas simiae WCS417)
MNSIFLRIYGGMCAALILVALLGVLALHLLNEVRSGQYRERLAHGTFALMGDNLQPMSPI
ERQRALAVWERLLGIPLELRPLADAQLDLSQRNRLQRGQVLVEQTGPHAARVLRLVNDQA
QLVLMGEVQQISEQLARATIYLLADELVRFPVAEQPQRLADIKEAKGFGFDMHLMTLDQA
DMDEDQRRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLELGPLYQMNPYPAQWLILITL
IGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGNLEVRVPARGADSVGRLAAAFNGMAE
HLQQLLAIQRELVRAVSHELRTPVARLRFGLEMVGDATTAEARRKYLEGMDSDIQDLDGL
VDEMLTYARLEQGSPELNFQRVDLDVLLDQVIGELSPLRPQVSVSRGICLSSAHWDDAWV
DAEPRYLHRALQNLVSNAMRHAQGHVLISYQVGQVRCRIDVEDDGPGVPESAWERIFTPF
LRLDDSRTRASGGHGLGLSIVRRIIYWHGGRALISKSNNLGGACFSLSWPRDQEKT