Protein Info for GFF4203 in Variovorax sp. SCN45

Annotation: Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 PF02629: CoA_binding" amino acids 15 to 107 (93 residues), 34.7 bits, see alignment E=6.3e-12 PF13380: CoA_binding_2" amino acids 17 to 141 (125 residues), 73.7 bits, see alignment E=4.4e-24 PF13607: Succ_CoA_lig" amino acids 163 to 301 (139 residues), 151.6 bits, see alignment E=2.9e-48 PF19045: Ligase_CoA_2" amino acids 315 to 397 (83 residues), 24.4 bits, see alignment E=6.8e-09 PF13549: ATP-grasp_5" amino acids 488 to 699 (212 residues), 218.4 bits, see alignment E=2e-68

Best Hits

KEGG orthology group: None (inferred from 87% identity to vap:Vapar_5497)

Predicted SEED Role

"Protein acetyltransferase" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (711 amino acids)

>GFF4203 Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative (Variovorax sp. SCN45)
MQTVEVGRGMDALFKPRSIAIFGASDDVTKIGGRPLQFLLKYGYAGGIYPINRKAGLVQS
LPAHANLDEVPEVPELAVVAVPPQGVLDAVRDCARRGVKAAVILSAGFSEMGDEGRRLQE
QIGEVARDTGMRVVGPNCLGAIGVPDKSIATFSVALESSFPAAGSVGIVSQSGNLGSFTL
RLAGERGLGVSRMLTTGNECDVDIADAIASLAADPDTRVILCCMETCRDGAKLIRAMAMA
REAGKPLVVLKVGVSEAGSEAAASHTGALAGSDAVFDAVLRNGGAIRVPSIEQLIEVGHA
LSVVGPAHVPGGNRVAVLTASGGFGVLLADAASAQGLALPKLAASTQERILSVVPFASPA
NPVDMTAQVSSRPEVLARMLDAVARDTSCDALILQSANAFHVPRLRDVFLSALAQVRKDH
PSRLILLCARAPQDVRAQLNGMGFPVVDGIDAACATVAALLRFGGGRGAAQNPVPAIERS
PLAAEAFATEASAKAVLAEAGLPVPREAIATSRDEALAAARAMGFPLVLKIVSPDIAHKT
EVGGVFIGVRSEAQLLEEYENLLARVALKAPEARIAGVLVAQMVHGGVELILGTKKDPVF
GPMVMVGLGGIFAEIFKDVALQPAPVDEAQATAMLRSLKAFALLDGARGRPRADVAAAAR
AVAALSRFATRHVQNVSEIDINPLMVLEQGHGAHALDALLVPEEQSMKANP