Protein Info for GFF4203 in Sphingobium sp. HT1-2

Annotation: DNA polymerase IV (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00817: IMS
  • amino acids 14 to 160 (147 residues), 180.4 bits, E=3e-57
• PF11798: IMS_HHH
  • amino acids 172 to 203 (32 residues), 32.4 bits, see alignment (E = 1e-11)
• PF11799: IMS_C
  • amino acids 246 to 351 (106 residues), 83.4 bits, E=2.4e-27

Best Hits

Swiss-Prot: 59% identical to DPO4_GLUOX: DNA polymerase IV (dinB) from Gluconobacter oxydans (strain 621H)

KEGG orthology group: K02346, DNA polymerase IV [EC: 2.7.7.7] (inferred from 81% identity to sch:Sphch_2172)

Predicted SEED Role

"DNA polymerase IV (EC 2.7.7.7)" in subsystem DNA repair, bacterial (EC 2.7.7.7)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Protein Sequence (365 amino acids)

>GFF4203 DNA polymerase IV (EC 2.7.7.7) (Sphingobium sp. HT1-2)
MDAPPPTLRKIIHIDMDAFYASVEQRDDPSLRGKAIAVGGGGPRGVVATCSYEARKFGVR
SAMPGARARRLCPELLFVKPRFEVYRSVSAQIRAIFARFTDMIQPLSLDEAYLDVTQNKP
GIASATQVAQEIRRLIQEETGLTASAGVSYNKLIAKLASDQNKPDGLCVVRPAEGADFMA
RMPVRRIHGIGPVTAERMHKFGIETGADLRDRDLSFLQTHFGSSALFYYRAARGEDDRPV
HERQERKSVSVEDTFFEDLVAEEALLREIERIAQSLWARIEKAQAYGRTVVLKVKFADFR
IITRSRSFAAPVRSVEQLVDAGRALLKAQLPLRMGARLLGLGVHNLEGEGEAGESGDAGA
QLSLL