Protein Info for GFF4199 in Sphingobium sp. HT1-2

Annotation: Altronate dehydratase (EC 4.2.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF08666: SAF" amino acids 25 to 95 (71 residues), 36.8 bits, see alignment E=6.9e-13 PF04295: GD_AH_second" amino acids 127 to 258 (132 residues), 133.1 bits, see alignment E=1.1e-42 PF20629: GD_AH_C" amino acids 270 to 508 (239 residues), 311.2 bits, see alignment E=6.5e-97

Best Hits

KEGG orthology group: K01685, altronate hydrolase [EC: 4.2.1.7] (inferred from 60% identity to pzu:PHZ_c2457)

Predicted SEED Role

"Altronate dehydratase (EC 4.2.1.7)" (EC 4.2.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.7

Use Curated BLAST to search for 4.2.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>GFF4199 Altronate dehydratase (EC 4.2.1.7) (Sphingobium sp. HT1-2)
MAYSKPMSILAETAPLLALQVAPEDDVAVALHPLGAGQTVRVGMVDVQLRDPVPAGHKFA
LHDIAADAPVRRYRAPIGLATRAILAGEHVHSHNLHTALSGELAYRFTGAQPQPLSPTDR
PVPHFRGYVRADGSVGTRNEIWILPTVGCVGRLGERLAMKGAALAEGRIDGVHAFNHPFG
CSQLGADLDGTAAILSSLALNPNAGGVLLLGLGCESNQLDALLSRIPPDRRARLRTVRAQ
SESDEFEAGMTALAELIDLASADQRQDVPLSALRLGVKCGGSDAMSGLTANPLIGRMADA
VAQAQGSAILTEIPEIFGAETLLMKRAENEAVFDRLTQVVNRFKRYFLDHGEPVSENPSP
GNIAGGITTLEEKSLGAVQKGGIAIVTDVIDYGDRISRTGLTILEGPGNDAVSTTALAAA
GATLTLFSTGRGTPLGSPVPTVKIASNSALADRKPGWIDFDAGRVLQEGMDAAADALLAH
IVAIASGAPARNEVNDERAIGIWKRGVTL