Protein Info for GFF4188 in Sphingobium sp. HT1-2
Annotation: Uncharacterized domain, not in CDD / GTP cyclohydrolase II (EC 3.5.4.25)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 86% identity to sch:Sphch_2209)Predicted SEED Role
"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)
MetaCyc Pathways
- flavin biosynthesis I (bacteria and plants) (8/9 steps found)
- flavin biosynthesis III (fungi) (7/9 steps found)
- 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
- toxoflavin biosynthesis (3/7 steps found)
- flavin biosynthesis II (archaea) (5/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12
Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (353 amino acids)
>GFF4188 Uncharacterized domain, not in CDD / GTP cyclohydrolase II (EC 3.5.4.25) (Sphingobium sp. HT1-2) MNGRDAARAIDALRRGWAIRLTAPDGAIRLMAIEGADAVTLADFDPQGQADILISAARAE TLKLANQLAAADPDLPVLIERAPWIDADVATSISDPVLDLASPLKGPFRARALPAPQAAK AALRLARLAGILPAYFLTEGDGPVEAEVSADDVGDYDDAIHLAIATRARLPVSASESAEI IAFRSPDEPREHVALVVGKRDASPPVIRIHSECLTGDVFGSLKCDCGPQLHQALHQIADA QWGVLLYLRQEGRGIGLVNKLRAYALQDQGFDTVDANVRLGFAIDARDFSVAARMLDLLG IGGVRLLTNNPQKVAGLQAAGIEVVERLPIILPANPHNERYLATKRDRTGHQL