Protein Info for GFF4187 in Xanthobacter sp. DMC5

Annotation: putative iron-sulfur-binding oxidoreductase FadF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 720 transmembrane" amino acids 22 to 44 (23 residues), see Phobius details amino acids 90 to 110 (21 residues), see Phobius details amino acids 129 to 152 (24 residues), see Phobius details amino acids 187 to 209 (23 residues), see Phobius details amino acids 221 to 241 (21 residues), see Phobius details amino acids 250 to 267 (18 residues), see Phobius details PF00037: Fer4" amino acids 315 to 334 (20 residues), 21.7 bits, see alignment (E = 4.2e-08) PF13183: Fer4_8" amino acids 317 to 402 (86 residues), 35 bits, see alignment E=5e-12 PF13187: Fer4_9" amino acids 319 to 401 (83 residues), 39.4 bits, see alignment E=1.5e-13 PF02754: CCG" amino acids 476 to 552 (77 residues), 21.1 bits, see alignment E=8.2e-08 amino acids 591 to 679 (89 residues), 51.2 bits, see alignment E=3.2e-17

Best Hits

KEGG orthology group: None (inferred from 91% identity to xau:Xaut_0918)

Predicted SEED Role

"Fe-S oxidoreductase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (720 amino acids)

>GFF4187 putative iron-sulfur-binding oxidoreductase FadF (Xanthobacter sp. DMC5)
MIPSGIDPSKVTRILFQDFSPWMLAIFYIYAIGCMAAFAWGVYAQIHKYRAGRRGDRLAW
GDIGRRLLDTAKTIASHRTLRRRDTAAGRLHAFIFYGFALLFIGTATITLQEDILGPLIG
LEFWHGHFYLLFKLTMTLAGTGFICGLLYMMWRRGWLKPPKLDYARPDRTPQDADFSRER
YRIEDWAFLWTLLLIGITGFLLSGARMVWLQNDPAVWDTRWWAPVGASIAAGLKAVGFTS
AGAGALRMGLWWLHGVLALTFIALIPYTKAKHIFTAAAAWLLRNPDAIRHLPLGDLDAAR
IGYRELGDVASRYLVQADACTKCGRCHEACPARAAGYPLSPRDVVLTLREQANTHLGEVL
PKPRAAGCATPLIGLATGEYRPETLWSCRQCGACTEICPVGVEHVPLINMLRRTLVDEGE
MDPALQRTLGAVNKTGNCFNESRRKRPNWTKDLPFKIKDARKEPVDVLWFVGDYASFDPR
NQKVSRAFARILHAAGIDFGILYEGETTAGNDVRRVGEEGLFQQLAEGNISTLGDCSFNR
IVTTDPHSFNTLKNEYPDLGGTYDVSHASAFLKELMADGRITPARKLDFTVTYHDPCHLG
RLNKGYDAPRDVLAATGVKLVEMGKSRDNSFCCGGGGGRVWVPDPPGTTKVGEIRAREAA
EIHGLDALVVNCPKCMTMLEDAVKTTGNERNFRVLELTELLAEALEPEEPALLPAPAEVG