Protein Info for GFF4186 in Xanthobacter sp. DMC5

Annotation: Trimethylamine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 PF00724: Oxidored_FMN" amino acids 14 to 344 (331 residues), 348 bits, see alignment E=1.6e-107 PF13450: NAD_binding_8" amino acids 399 to 438 (40 residues), 24.7 bits, see alignment 5.8e-09 PF22620: OYE-like_second_a-b" amino acids 521 to 648 (128 residues), 240.9 bits, see alignment E=7.2e-76

Best Hits

Swiss-Prot: 72% identical to DHTM_METME: Trimethylamine dehydrogenase (tmd) from Methylophilus methylotrophus

KEGG orthology group: None (inferred from 97% identity to xau:Xaut_0917)

MetaCyc: 72% identical to trimethylamine dehydrogenase subunit (Methylophilus methylotrophus W3A1)
Trimethylamine dehydrogenase. [EC: 1.5.8.2]

Predicted SEED Role

"Histamine dehydrogenase"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.8.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (735 amino acids)

>GFF4186 Trimethylamine dehydrogenase (Xanthobacter sp. DMC5)
MTHETLGVSKYDILFEPIKIGPKTLRNRFYQVPHCIGAGSDKPGFQAAHRSIKAEGGWAA
LNTEYCSIHPESDDTHRVSARIWDEGDVRNLAAMTSEIHKYGALAGVELWYGGAHAPCME
TRQTARGPSQYASEFESLTYCHEMDHDDIRRVQGFYVEAARRARDAGFDIIYCYGAHSYL
PLQFLSKYYNKRTDKYGGSFENRARFWIETLEQMKAAVGSECAIATRFAVDTLIGEDGIE
VERDGLKFVELADDLVDLWDVNVGDIAEWGEDAGPSRFYRQGHQLSWQKFVKQASKKVVL
GVGRFTDPEKMVEVIRNGELDVIGAARPSIADPFLPEKIRDGRLDDIRTCIGCNVCISRW
EIGGPPMICTQNATAGEEFRRGWHPERFPKKGSEDAILVVGAGPSGSECARVLMERGYVV
HLVDSAEKIGGYVNEVATLPGLGEWSYHRDYREVQLNKLVKRNRDSQIALGGKKLSVEDV
LSYGADKVIVATGAHWVTDGTNCLTHAPIPGLEGTPRANVLTPEDVILGTKPIGKRVMIL
NADPYYMAPSLAQKLAEAGHQVTVASGVELGRYMHFTLEAPNMHRMLHELHINVLSSTWA
SRVEDGRIELYNLWGDGHKRVYTGPGKMPRSANTTHAWHEYDTLVLVTARRSEDALFRAL
KARSGEWDARGIKGVYVIGDAWAPKLIADATFDGQRLAREIEEANPQEPKPYRRESAVYG
APYQPGGSYEIRYQG