Protein Info for GFF4165 in Xanthobacter sp. DMC5

Annotation: NADP-dependent 3-hydroxy acid dehydrogenase YdfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08659: KR
  • amino acids 10 to 166 (157 residues), 33.3 bits, E=9.6e-12
• PF00106: adh_short
  • amino acids 11 to 193 (183 residues), 162.7 bits, E=1.6e-51
• PF01370: Epimerase
  • amino acids 12 to 147 (136 residues), 23.5 bits, E=6.9e-09
• PF13561: adh_short_C2
  • amino acids 15 to 225 (211 residues), 99.9 bits, E=3.4e-32

Best Hits

Swiss-Prot: 54% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: None (inferred from 82% identity to xau:Xaut_2812)

MetaCyc: 53% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"short-chain dehydrogenase/reductase SDR( EC:1.1.1.276 )"

MetaCyc Pathways

• 3-hydroxypropanoate cycle (10/13 steps found)
• L-threonine degradation III (to methylglyoxal) (2/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• aminopropanol phosphate biosynthesis II (2/4 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• glyoxylate assimilation (8/13 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Protein Sequence (257 amino acids)

>GFF4165 NADP-dependent 3-hydroxy acid dehydrogenase YdfG (Xanthobacter sp. DMC5)
MAMLDPRTLTLVVTGATSGIGAATVRRFASAGSKVVAVGRRADRLEGLKAEFGDLILPLE
LDVRDEKATFDAFSSLPAPFADYNIVFANAGLALGLEPAQKADLEEWKTMVETNITGMLT
TVRASLPAMVARKEGHVVFTGSVAGDYPYFGGNVYGATKAFVKQFSLNLWADVAGTGVRV
TNIEPGLVETEFSVVRFAGDKGKADQVYAGYTPMTGEDIAEQVYFACTMPRHVNINRIQC
MAGQQTFNGLTVRRDPA