Protein Info for GFF416 in Sphingobium sp. HT1-2

Annotation: beta-glucosidase (EC 3.2.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00933: Glyco_hydro_3
  • amino acids 59 to 375 (317 residues), 213.1 bits, E=1.1e-66
• PF01915: Glyco_hydro_3_C
  • amino acids 416 to 650 (235 residues), 171.1 bits, E=4.7e-54
• PF14310: Fn3-like
  • amino acids 687 to 756 (70 residues), 73.4 bits, E=2e-24

Best Hits

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 82% identity to sjp:SJA_C1-08060)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

• cellulose degradation II (fungi) (2/3 steps found)
• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Phenylpropanoid biosynthesis
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Protein Sequence (778 amino acids)

>GFF416 beta-glucosidase (EC 3.2.1.21) (Sphingobium sp. HT1-2)
LSSDKPRSFFPGYALSRMTLDRRDLLIRTLGGVLALGIPRRALAQADVARVDALIAKMTL
EEKAGQMTCLADSFRPFNPPNPAAGIQDEKRLADEIRKGRVGCLFNGIGVAGGRRAQEMA
VKNSRLGIPLLLAGDVIHGLKTIFPVPLGEASCFDPVLVEKTARVMAVEATAAGLHLTFA
PMVDVARDQRWGRVVEGAGEDVYLTGLLSAARIRGFQGRDLRRDDSLLACPKHFAAYGAV
AAGLEYGSVDISDETLRETHLPPFGKAFAAGALTTMAAFNEINGVPATADRELLTDILRG
EMQFRGFVFSDYTADEELVAHGFAEDERDAARLAVLAGVDMSMQSGLYIRHIPDLVKSGA
VPMATVDVAVRRILYVKTAMGLFDNPWRSLDEEVEKARIGTPAHHALAREAAARSVVLLK
NDGVLPLDPAAGQRIALIGPFGEDKANLYGPWAFYGDPDKGVDIATGLRAAMPDPAKLTV
ELGCAVNGPINEGLARAVAAAKAADVVILAVGESQAMSGEAQSRTMIELPSSQQALADAI
AATGKPVIVLLRHGRALALHDGVANAQALLATWFLGSETGHALADILFGKVDPSAKLPVS
FPWESGQEPFFYDRKSTGRPTVDNGSTEYKARYATTDNSARFPFGHGLSYTDFALSDLKL
SDTALRWDGAVEITARLTNKGKRKGSEVVQLYIRDRVASRTRPIRELKRIQRVTLSPGDS
TQVRFTLSRTDLEFVGAGNRRLAEPGLFDLWVGQSSTGGLHGQFTLYAAAPASPPAKG