Protein Info for GFF4146 in Sphingobium sp. HT1-2

Annotation: Leucyl-tRNA synthetase (EC 6.1.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 TIGR00396: leucine--tRNA ligase" amino acids 9 to 837 (829 residues), 1023.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 225 (212 residues), 95.1 bits, see alignment E=1e-30 amino acids 419 to 575 (157 residues), 29.2 bits, see alignment E=1.1e-10 amino acids 612 to 651 (40 residues), 36.1 bits, see alignment 8.3e-13 PF09334: tRNA-synt_1g" amino acids 40 to 171 (132 residues), 68.1 bits, see alignment E=1.6e-22 amino acids 612 to 654 (43 residues), 27.2 bits, see alignment 4.3e-10 PF13603: tRNA-synt_1_2" amino acids 221 to 405 (185 residues), 213.2 bits, see alignment E=5.5e-67 PF08264: Anticodon_1" amino acids 682 to 801 (120 residues), 63.8 bits, see alignment E=4.2e-21

Best Hits

Swiss-Prot: 75% identical to SYL_SPHWW: Leucine--tRNA ligase (leuS) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 85% identity to sch:Sphch_2242)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (837 amino acids)

>GFF4146 Leucyl-tRNA synthetase (EC 6.1.1.4) (Sphingobium sp. HT1-2)
MQRRFNPLEADARWQAVWDEKQSFKADDASTKPRSYVLEMFPYPSGRIHIGHVRNYSMGD
VLARFRRMTGHEVLHPMGWDAFGMPAENAAMEKKVHPGEWTRSNIANMRAQLKKLGFAID
WSRELATCEPDYYGHEQALFLDMLESGLVYRKESQVNWDPVDMTVLANEQVIDGRGWRSG
ALVEKRKLNQWFLKITQFADDLLEGLQTLDQWPDKVRLMQENWIGKSVGLQFSFKPVAPF
DSEIEVYSTRPDTIFGASFVAIAADHPVAQAVAANNPDAVAFIEKCKEGGTTAAELETAE
KLGFDTGLTVAHPFDPNWHLPVFIANFVLMDYGTGAVMGVPAHDQRDLDFARKYMLPVER
VVALDGEADKPIYDEAYTGPGNLVNSRFLDGMAVEAAKAAVIARAEGEGWGAGKTVFRLR
DWGVSRQRYWGTPIPVIHCDDCGVVGVPKDQLPVKLPEDVTFDIPGNPLDRHPSWKHVDC
PSCGKAARRETDTLDTFADSSWYFIRFASQPDDKPFDRATVEQWLPVGQYIGGVEHAILH
LLYARFWTRALQHMGQLGFAEPFTGLFTQGMVTHETYKAGDGSWLSPQEIKKSGDDYIHI
ESGAPVTVGRVEKMSKSKKNVVDPDDIIEQYGADAVRWFMLSDSPPERDLPWTEAGIEGS
WRFVNRVWRLFGEADAAAEGQDKALDRKLHQTIDGVAKDIEALGFNKAVAKIYELVNAIE
KAKPSASRTAAIRALALLVAPMTPHLAEEGWAEMAQPGLIAEAAWPAVDPALLVEDEVTI
ACQVMGKLRDTITVPKGTPKDELEKLALAAPNVVRTLDGATPKKVIVVPDRLVNLVI