Protein Info for GFF4132 in Sphingobium sp. HT1-2

Annotation: tRNA-5-carboxymethylaminomethyl-2- thiouridine(34) synthesis protein MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 616 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 5 to 613 (609 residues), 725 bits, see alignment E=3.5e-222 PF07992: Pyr_redox_2" amino acids 5 to 155 (151 residues), 34.6 bits, see alignment E=4.3e-12 PF01134: GIDA" amino acids 6 to 394 (389 residues), 505.8 bits, see alignment E=2.6e-155 PF00890: FAD_binding_2" amino acids 6 to 41 (36 residues), 20.5 bits, see alignment 7.6e-08 PF12831: FAD_oxidored" amino acids 6 to 154 (149 residues), 34.3 bits, see alignment E=5.1e-12 PF21680: GIDA_C_1st" amino acids 459 to 547 (89 residues), 35.8 bits, see alignment E=3.5e-12 PF13932: SAM_GIDA_C" amino acids 553 to 608 (56 residues), 67.4 bits, see alignment 2.3e-22

Best Hits

Swiss-Prot: 63% identical to MNMG_SPHAL: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 84% identity to sjp:SJA_C1-00290)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (616 amino acids)

>GFF4132 tRNA-5-carboxymethylaminomethyl-2- thiouridine(34) synthesis protein MnmG (Sphingobium sp. HT1-2)
MRTSYDVIVVGGGHAGCEAAAAAARKGAAVALLTFERATVGAMSCNPAIGGLGKGHLVRE
VDALDGLIGRAADAGAIHYRMLNSSKGAAVQGPRVQADRKRYKAAIHQMLDAQAGLDIVE
GEAVGLIVREGTVAGLALADGRTLEAPSVVLATGTFLGGKLFRGEDVDVGGRTGERAATA
LGVQLREMGLPIGRLKTGTPPRIDGRTIDWAVLEEQASDGEGWTMSPLSDHRPLPQLACA
ITRTNERTHAIIREGLGRSPLFSGAIEGRGPRYCPSIEDKVIRFGDRDGHQIFLEPEGLD
DALVYPNGISTSLPADVQQAMIRSMRGLEQAEIVVPGYAVEYDHIDPRALDASLEVRALP
GLFCAGQINGTTGYEEAAAQGLVAGTNAAARARSEAPMILDRASSYIGVMIDDLVLQGVT
EPYRMLTARAEYRLRLRADNAETRLGEIGRTHGIIGPDRAARFAARASARAAIEAELARP
FTATEMARAGAAVRQDGARRSLFEWARFPEVQAELLVTLAPNLAEVPADLRAEILEDAHY
APYLERQDAEIAALRRDERVAIPADFDFASVGGLSTEMIERLDGARPDTLAAAGRVRGIT
PAALAAILVHVRRKAA