Protein Info for GFF4131 in Sphingobium sp. HT1-2

Annotation: 16S rRNA (guanine(527)-N(7))-methyltransferase (EC 2.1.1.170)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 PF02527: GidB" amino acids 23 to 191 (169 residues), 117.2 bits, see alignment E=2.9e-38 TIGR00138: 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG" amino acids 24 to 182 (159 residues), 113.3 bits, see alignment E=5.5e-37

Best Hits

Swiss-Prot: 50% identical to RSMG_SPHAL: Ribosomal RNA small subunit methyltransferase G (rsmG) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03501, ribosomal RNA small subunit methyltransferase G [EC: 2.1.1.170] (inferred from 78% identity to sch:Sphch_2256)

Predicted SEED Role

"rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.170

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (213 amino acids)

>GFF4131 16S rRNA (guanine(527)-N(7))-methyltransferase (EC 2.1.1.170) (Sphingobium sp. HT1-2)
MTEDEARAWLDAHFDVPRETWDRLERYVSLLLSAMDEQNLIAESTRPHVWARHIVDSAQL
LLHAKDAGEGPWMDLGSGAGLPGIVVASMSDRPLIMVESRRKRIDFLNDVIAELGLTNAS
VFGGRVEVAPPTKSAVISARAYAPLPKLLQSAQHLSDEKTIWVLPKGRNAQNELETARPA
WQGAFHVERSVTDADSAIIVARSVKPVKKKGRG