Protein Info for GFF4128 in Sphingobium sp. HT1-2

Annotation: Membrane alanine aminopeptidase N (EC 3.4.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 867 TIGR02414: aminopeptidase N" amino acids 19 to 865 (847 residues), 1160.9 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 90 to 195 (106 residues), 46.2 bits, see alignment E=1.2e-15 PF01433: Peptidase_M1" amino acids 235 to 445 (211 residues), 177.8 bits, see alignment E=4.9e-56 PF11940: DUF3458" amino acids 453 to 546 (94 residues), 96 bits, see alignment E=2.8e-31 PF17432: DUF3458_C" amino acids 551 to 866 (316 residues), 395.2 bits, see alignment E=5e-122

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 85% identity to sjp:SJA_C1-00250)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.2

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (867 amino acids)

>GFF4128 Membrane alanine aminopeptidase N (EC 3.4.11.2) (Sphingobium sp. HT1-2)
MADIQSTQAAAPAIIRRADYRVPDWLIPDIALDFDLDAARTRVWSDMSVARNGDHDRPLR
LDGDGLVPLAVKVDGRTLNETEWTLEGGALVVSLGGATHKVEVLVELAPESNSKLMGLYA
SGGLLCTQCEAEGFRRITFFPDRPDILSRYSVKLTADKARYPILLANGDPVKQGDLEDGR
HWAQWNDPFPKPCYLFALVAGDLACNADRFVTMSGREVALGIWVHEADLPRTDHAMQALK
NSMAWDERVYGREYDLDVFNIVAVADFNFGAMENKGLNIFNSRYILADPETATDIDYDGV
EGVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDQNFSADMGSHAVKRIEDVRILRAA
QFQEDSGPLAHPVRPESYMEISNFYTATIYNKGAELIRMMALMLGAERFRAGTDLYFDRH
DGEAATCEDFVRAMEEGGEIDLGQFRRWYEQAGTPHVRALLSHDPVSRTAELLLEQSVPP
TPGQPDKRPMAIPLRVALYDPATGSHHGDELLMLTEAQQRFTFDNFASLPILSINRGFSA
PVIVETNRSQADLAFLSAHDDDPFARYEAMQQLMVNVLVGQVAGQSVDVTAVIDAVRNTI
TDPLLDPAFVAEAVRLPSEAYLGDQMKQVDPDAIHAARDALQTRLGADLEPLWRDIHART
KANGFAVSPAAKGARKLRNVALIYLVASGAEDGPAIAYGQFSEADNMTERQSALATLASG
TSPEREAALDIFYNRYSDDALTLDKWFQTQAFAFHTDTVELVAQLGQHKAFTLNNPNRVR
SLYGAFAGNQWAFHHKSGKGYRLVADCIIALDKLNPQTAARLVPPLGRWKRFDEGRAALM
RAELQRILDEPGLSKDVTEQASKSLEG