Protein Info for GFF4117 in Variovorax sp. SCN45

Annotation: Translation elongation factor Tu

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 TIGR00485: translation elongation factor Tu" amino acids 4 to 397 (394 residues), 532 bits, see alignment E=7.7e-164 PF00009: GTP_EFTU" amino acids 6 to 203 (198 residues), 187.5 bits, see alignment E=3.9e-59 TIGR00231: small GTP-binding protein domain" amino acids 8 to 138 (131 residues), 46.5 bits, see alignment E=3.4e-16 PF01926: MMR_HSR1" amino acids 10 to 131 (122 residues), 22.4 bits, see alignment E=2.2e-08 PF03144: GTP_EFTU_D2" amino acids 227 to 297 (71 residues), 62.5 bits, see alignment E=8.6e-21 PF03143: GTP_EFTU_D3" amino acids 304 to 396 (93 residues), 100.8 bits, see alignment E=1e-32

Best Hits

Swiss-Prot: 58% identical to EFTU_CALBD: Elongation factor Tu (tuf) from Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320)

KEGG orthology group: K02358, elongation factor Tu (inferred from 58% identity to cow:Calow_0656)

Predicted SEED Role

"Translation elongation factor Tu" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (398 amino acids)

>GFF4117 Translation elongation factor Tu (Variovorax sp. SCN45)
MMNNKVHVNVGTIGHVDHGKTTLTAALTFVQAARFGGDALSYGQIDNAKEERERGITINT
SHVEYESDRRHYAHIDCPGHADYIKNMITGASQMDGAILLVDGTEGAARQTIEHVLLARQ
VNVGHMVVFVNKMDLVADGDRADIEQLIEMEIGALLAAHGYHDACFVFGSALKALVAAER
GDLEGAEVQCIVELVNALDSRIPDPVRDYDAPFMMPVEGVHTITGRGTVISGRVERGVIR
VGDNLEIVGLSAAGDEKVVVTGVQSFHKDVAEARAGMNVGLLLRGVKRDDVVRGQVVVKP
GALKPYRTGRAQIFVLSKDEGGRHTPFGSGYQPQFFFGTTDVTGVIRVDTDTGMVEPGAQ
AQIGFELNKPVAIENGMRFAMREGGKTVGAGIVTEVLS