Protein Info for GFF4114 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 5 to 365 (361 residues), 519.2 bits, E=3.1e-160
• PF00696: AA_kinase
  • amino acids 6 to 235 (230 residues), 161.6 bits, E=2.7e-51
• PF01472: PUA
  • amino acids 276 to 350 (75 residues), 74.8 bits, E=4.3e-25

Best Hits

Swiss-Prot: 100% identical to PROB_SALA4: Glutamate 5-kinase (proB) from Salmonella agona (strain SL483)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 100% identity to spq:SPAB_03279)

MetaCyc: 97% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (367 amino acids)

>GFF4114 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDT
LRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLFTADPR
SNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPG
VIGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGI
KSVTGNFSRGEVIRICNLQGRDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVH
RDDMITR