Protein Info for GFF4110 in Xanthobacter sp. DMC5

Annotation: 3-oxo-isoapionate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 PF07005: SBD_N" amino acids 17 to 253 (237 residues), 179.4 bits, see alignment E=9.8e-57 PF17042: NBD_C" amino acids 279 to 452 (174 residues), 138.7 bits, see alignment E=2.8e-44

Best Hits

Swiss-Prot: 68% identical to OIAK_PARG4: 3-oxo-isoapionate kinase (oiaK) from Paraburkholderia graminis (strain ATCC 700544 / DSM 17151 / LMG 18924 / NCIMB 13744 / C4D1M)

KEGG orthology group: None (inferred from 77% identity to xau:Xaut_2923)

MetaCyc: 68% identical to 3-oxoisoapionate kinase (Paraburkholderia graminis C4D1M)
RXN-20934 [EC: 2.7.1.231]

Predicted SEED Role

"Predicted pyridoxine biosynthesis protein (probably from glycolaldehide)"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.231

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>GFF4110 3-oxo-isoapionate kinase (Xanthobacter sp. DMC5)
MISGTKTGAALPEGLLLAYYGDDFTGSTDALEAITAAGVPSVLFLRPPTEEMRARFPHAR
AIGLAGSSRGRSPEWMDKELPPLFARLAALGAPVLHYKVCSTFDSAPHVGSIGRAIDLGV
KQMGGAWSPMVVGVPRLGRYQMFGNLFVTWNGTTYRLDRHPTMSRHPVTPMDEADLSRHL
ARQTDRRIALIDLAEIRAGKAQARCETLRGDDTPVVLIDVMDEASLVEAGRLIWENRGDG
LFSASSSGLQYALAAYWRSLGLLPETPGLPKAVPVEKIAVVSGSCSPVTAAQLAHARAAG
FHTERLDLPRALSDADGQAEIARAVASAMDALSRGQSPVVYSAEGPDDAAVTGFDATAAA
AGLSRADAARRVGTVLAEIMRRIVESGAVRRVMVAGGDSSGEVMEAMNIFALDIVAGLAP
GAPLCRAWSDDALYNGLELVLKGGQMGPTDFFPQVRG