Protein Info for GFF4106 in Variovorax sp. SCN45

Annotation: ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 PF00005: ABC_tran" amino acids 35 to 185 (151 residues), 130.5 bits, see alignment E=1e-41

Best Hits

Swiss-Prot: 51% identical to TCYC_BACSU: L-cystine import ATP-binding protein TcyC (tcyC) from Bacillus subtilis (strain 168)

KEGG orthology group: K02028, polar amino acid transport system ATP-binding protein [EC: 3.6.3.21] (inferred from 88% identity to vap:Vapar_1886)

MetaCyc: 54% identical to cystine ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"ABC-type polar amino acid transport system, ATPase component"

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.21

Use Curated BLAST to search for 3.6.3.21 or 7.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (287 amino acids)

>GFF4106 ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines) (Variovorax sp. SCN45)
MPATDNATDNAANPAPFPLVALRGVQLAFGDNAVLRGIDLDVQRGQAVSIIGPSGSGKST
ILRCITGLLRPQQGEIRVGDTRVDTLRTEAELIALRKRVGFVFQQYNLFPHLTVLQNLVI
APTRVAGRDRATAERDARALLDKVRLSHKETAYPGELSGGQQQRVAIARALAMRPELMLF
DEVTSALDPETVGEVLTVIRDLVRDGLTCVLVTHEMRFAEEISDTVYFTEAGVIVEHGPA
ARIFGAPESERTRAFLHRALGEGPRPAPARPAPIPETLSFERLRFAL