Protein Info for GFF4078 in Xanthobacter sp. DMC5

Annotation: (2R)-sulfolactate sulfo-lyase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 79% identical to SUYB_PARPN: (2R)-sulfolactate sulfo-lyase subunit beta (suyB) from Paracoccus pantotrophus

KEGG orthology group: K01685, altronate hydrolase [EC: 4.2.1.7] (inferred from 88% identity to mrd:Mrad2831_5979)

MetaCyc: 79% identical to 3-sulfolactate sulfo-lyase large subunit (Paracoccus pantotrophus NKNCYSA)
Sulfolactate sulfo-lyase. [EC: 4.4.1.24]

Predicted SEED Role

"Altronate dehydratase (EC 4.2.1.7)" (EC 4.2.1.7)

MetaCyc Pathways

D-galacturonate degradation I (4/5 steps found)
(R)-cysteate degradation (2/3 steps found)
sulfolactate degradation I (2/3 steps found)
superpathway of hexuronide and hexuronate degradation (7/10 steps found)
superpathway of microbial D-galacturonate and D-glucuronate degradation (15/31 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.7

Use Curated BLAST to search for 4.2.1.7 or 4.4.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (426 amino acids)

>GFF4078 (2R)-sulfolactate sulfo-lyase subunit beta (Xanthobacter sp. DMC5)
MSNIAMFHAQTAPNPFRGGAPDQLITKATPTALASADYANATVRGWRRENGRVGVRNHVV
ILPLDDLSNAACEAVAAHVKGTMALPHAYGRLQFGEDLEIHFRTLIGIGSNPNVAAVVVI
GIEDQWTNRVVEGIAKTGKPVVGFGIELNGDIATVAKASYEAKRFVQWANELPQEDCPLS
DLWISTKCGESDTTTGLSSCPTVGNMYDKLIPHGIYGVFGETSEITGAEHLCKERAATPE
VAEKWFKMWKSYQDDVIEAHKTDDLSDSQPTKGNIAGGLTTIEEKALGNLEKIGRECRYI
DALEPAEAPAKGPGLYYMDTSSAAAECVTLMAAAGYVIHTFPTGQGNVIGNPIVPVIKIS
GNPRTIRTMGEHIDVDVSGVLRREMTIPEAGDALIDMIVRTANGRLTAAESLGHREFVMT
KLYRSA