Protein Info for GFF4060 in Xanthobacter sp. DMC5

Annotation: Phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01515: PTA_PTB
  • amino acids 4 to 324 (321 residues), 390.1 bits, E=4.5e-121
• TIGR00651: phosphate acetyltransferase
  • amino acids 18 to 323 (306 residues), 354.2 bits, E=3.5e-110

Best Hits

Swiss-Prot: 47% identical to PTAS_CLOTH: Phosphate acetyltransferase (pta) from Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)

KEGG orthology group: K00625, phosphate acetyltransferase [EC: 2.3.1.8] (inferred from 67% identity to smk:Sinme_4739)

MetaCyc: 44% identical to phosphate acetyltransferase (Clostridium acetobutylicum)
Phosphate acetyltransferase. [EC: 2.3.1.8]

Predicted SEED Role

"Phosphate acetyltransferase (EC 2.3.1.8)" in subsystem Ethanolamine utilization or Fermentations: Lactate or Fermentations: Mixed acid or MLST or Propanediol utilization or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or Threonine anaerobic catabolism gene cluster (EC 2.3.1.8)

MetaCyc Pathways

• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• acetyl-CoA fermentation to butanoate (6/7 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• ethanolamine utilization (4/5 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• heterolactic fermentation (13/18 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate IV (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• sulfoacetaldehyde degradation I (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• acetylene degradation (anaerobic) (3/5 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• sulfolactate degradation II (1/4 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)
• L-lysine fermentation to acetate and butanoate (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• methanogenesis from acetate (2/6 steps found)
• superpathway of sulfolactate degradation (2/6 steps found)
• superpathway of taurine degradation (2/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (4/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)
• superpathway of methanogenesis (5/21 steps found)
• superpathway of L-lysine degradation (13/43 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.8

Use Curated BLAST to search for 2.3.1.8

Protein Sequence (359 amino acids)

>GFF4060 Phosphate acetyltransferase (Xanthobacter sp. DMC5)
MLALDRIIDAARAAPRNIVLPEGSDPRIAAGARRAAREGIARPILLGAREEVMAALDGDA
RGIAIEDPATSPRLQAYAEALHLLRHAKGMDLATAWRRVCEPLAFAAMMVRQGDADGTVG
GAVATTAETVRAALQIIGLAPGAKIVSSFFLMILCEDYHEQKGAYVFSDCGLVIDPDAAE
LADIARSAAHSYTAMTGEAPRVAMLSFSTNGSARHQRVDKVVEAVRLVREAEPDLVIEGE
LQFDAAFVATVGAAKTAPGSRIRGDANVFVFPNLDAANIGYKIAQRIGGAKAIGPVLQGL
AKPANDLSRGCSQDDVFHMIALTCAQAGRTLPVAMSGKIDSVWQNSRPAAASLGETRAG