Protein Info for GFF4050 in Sphingobium sp. HT1-2

Annotation: Cell division trigger factor (EC 5.2.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 531 PF05697: Trigger_N" amino acids 2 to 142 (141 residues), 128 bits, see alignment E=5.5e-41 TIGR00115: trigger factor" amino acids 11 to 419 (409 residues), 432.2 bits, see alignment E=9.2e-134 PF00254: FKBP_C" amino acids 159 to 240 (82 residues), 66.9 bits, see alignment E=2.4e-22 PF05698: Trigger_C" amino acids 264 to 421 (158 residues), 117 bits, see alignment E=1.4e-37

Best Hits

Swiss-Prot: 67% identical to TIG_SPHAL: Trigger factor (tig) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03545, trigger factor (inferred from 88% identity to sch:Sphch_1016)

Predicted SEED Role

"Cell division trigger factor (EC 5.2.1.8)" in subsystem Bacterial Cell Division (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (531 amino acids)

>GFF4050 Cell division trigger factor (EC 5.2.1.8) (Sphingobium sp. HT1-2)
MQTVETLNEGLKRAYTVTITSKDIDARVEKEVLAIAPQVRMPGFRPGKVPANLVKKMHGE
SLQRDALNNSIQESVQKLIADQKLRPAMQPSVELDESFEFGKDAQVKVELEVLPVIEAPS
IDGLKLERLTAEVSDAQVEEQAGRIATQQGALEEYAASHKAKDGDTLVIDFVGKVDGEAF
EGGSAEGVKLKIGSGQFIPGFEEQLGGVKKGEEKTIEVTFPEDYGAAHLAGKAATFDVTV
QSVLNADKPKLDDDFAKSLGLEGLDQLKDLLKGQIEQEHNGLTRTYMKRKLLDQLAASHD
FDVPPSMVEAEFNQIWQQLQHEASHEADPEAALAEMEAEKEDYRGIAVRRVRLGLLLSEI
GQANNVVVSDNEMNRLIMQAAQQYSPQDRERFVQYVRQDPMAAAQLRAPLYEDKVVDFLF
EKAEVSDRAVSREELEAAIEAEDGDLKPHVHGPGCGHDHDHDDKPKAKKAAAKKKAEPAE
AEAVAEEAPAKKAPAKKAAAKKDEAPAEEAVAEEKPKAKKAPAKKAAAKAE