Protein Info for GFF405 in Sphingobium sp. HT1-2

Annotation: Para-aminobenzoate synthase, aminase component (EC 2.6.1.85)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00553: aminodeoxychorismate synthase, component I
  • amino acids 60 to 389 (330 residues), 376.3 bits, E=6.2e-117
• PF00425: Chorismate_bind
  • amino acids 130 to 385 (256 residues), 288.5 bits, E=2.6e-90

Best Hits

KEGG orthology group: K01665, para-aminobenzoate synthetase component I [EC: 2.6.1.85] (inferred from 78% identity to sjp:SJA_C1-08130)

Predicted SEED Role

"Para-aminobenzoate synthase, aminase component (EC 2.6.1.85) / Aminodeoxychorismate lyase (EC 4.1.3.38)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Folate Biosynthesis (EC 2.6.1.85, EC 4.1.3.38)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• 4-aminobenzoate biosynthesis II (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of chorismate metabolism (41/59 steps found)
• superpathway of candicidin biosynthesis (3/11 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.85, 4.1.3.38

Use Curated BLAST to search for 2.6.1.85 or 4.1.3.38

Protein Sequence (392 amino acids)

>GFF405 Para-aminobenzoate synthase, aminase component (EC 2.6.1.85) (Sphingobium sp. HT1-2)
MRLPGPTEAFALFDDARVQGAAPARLYRDPVSVIAAWRMEEVQPALDRIAEAREAGLHAA
GYISYEAGLALEERLAPIAHRPPKGGPQAMPLLWFGLFEGCRFIPADGVADLLPDPAAVS
VDAPWPLVSEADYRAQFDQVQELIRAGDIYQVNLTFPCELRFAGDPMALYAALRPRAQAG
YGGIINIGGHQILSFSPELFFTQVRGQLTARPMKGTAVRDHDPDRDAALATELARDAKQR
AENLMIVDLLRNDLSRVARAGSVTVPDLFKVETYPTVHQMVSTIRGRVLPGLAPVDVLRV
LFPCGSITGAPKVRAMEIIDMVEPFARGVYTGAMGWMDPAGDAAFNVAIRTICVEEGSGI
GRLGLGSGIVADSDASAEWAECLAKGRFLSLG