Protein Info for GFF404 in Methylophilus sp. DMC18

Annotation: Sulfate/thiosulfate import ATP-binding protein CysA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 TIGR00968: sulfate ABC transporter, ATP-binding protein" amino acids 3 to 242 (240 residues), 407.6 bits, see alignment E=8.1e-127 PF00005: ABC_tran" amino acids 18 to 164 (147 residues), 138.5 bits, see alignment E=3.5e-44 PF12857: TOBE_3" amino acids 263 to 320 (58 residues), 57 bits, see alignment E=2.3e-19

Best Hits

Swiss-Prot: 62% identical to CYSA_PSEAE: Sulfate/thiosulfate import ATP-binding protein CysA (cysA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02045, sulfate transport system ATP-binding protein [EC: 3.6.3.25] (inferred from 66% identity to lch:Lcho_3807)

Predicted SEED Role

"Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25)" in subsystem Cysteine Biosynthesis (EC 3.6.3.25)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.25

Use Curated BLAST to search for 3.6.3.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (325 amino acids)

>GFF404 Sulfate/thiosulfate import ATP-binding protein CysA (Methylophilus sp. DMC18)
MSITIQQINKGFGQFSALRDINLQVPSGELVALLGPSGCGKTTLLRIIAGLETPDSGQVL
FDGVDTTHRDVRERQVGFVFQHYALFRHMTVFENVAFGLRVRPKETRPSEAEIKKRVHDL
LKLVQLDWLADRYPPQLSGGQRQRIALARALAVEPKVLLLDEPFGALDAKVRKDLRRWLR
RLHDELHVTSVFVTHDQEEALEVADTIVVMNHGQVEQVGSPQAVYDNPATPFVYEFLGNV
NAFETPQGKGFVRPYEIAVSREADAQSIAGALTYVHSVGSLVRLEIKRADNEQYVDVELG
REQFNQLSFKQGETVYLKPTQLRVF