Protein Info for GFF4038 in Variovorax sp. SCN45

Annotation: Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 74 to 393 (320 residues), 166.3 bits, see alignment E=4.3e-53 PF16576: HlyD_D23" amino acids 81 to 315 (235 residues), 175.3 bits, see alignment E=1.7e-55 PF13437: HlyD_3" amino acids 212 to 311 (100 residues), 63.3 bits, see alignment E=5e-21

Best Hits

KEGG orthology group: None (inferred from 54% identity to dar:Daro_2253)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>GFF4038 Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family (Variovorax sp. SCN45)
MKFNSALVDQRSTERSALTKLAAAICAAFAVIALSGCGGSNGSAEKPAVEEKAAEKEGLK
LSKEEADRAGIVLEELKAQALADTVTVTATIRANQDRIARIAPRVEGRVATVAASLGDNV
RTGQSLATLDSLAVGEASSALSQARSAQRVADADYKRATALQAEEIIPQKDYLRAKAEHE
KASATLRAAEDHLRLLGVPSGQSAGVTSVFPLTSPLAGTIIEKKAVVGGLAGPSDALFVV
ADLSTLWIEANLAEAQLAKVRVGAKATVTVGAYPDERFEGRVTYVASILDKETRSIPARI
EVKNGEGRLKPEMFATATIESPSPQSAPKTDVLTVPDQAIVLMQGQPNVFVFENGAYEQR
AIDPGDRLGTRTVVKSGVKAGEQVVSAGTYALKARVLKSQIGDAH