Protein Info for GFF4035 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: D-amino acid dehydrogenase small subunit (EC 1.4.99.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 PF01266: DAO" amino acids 1 to 398 (398 residues), 236 bits, see alignment E=2.3e-73 PF13450: NAD_binding_8" amino acids 3 to 32 (30 residues), 26.8 bits, see alignment (E = 1.3e-09)

Best Hits

Swiss-Prot: 48% identical to DADA2_RALSO: D-amino acid dehydrogenase 2 (dadA2) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 64% identity to vpe:Varpa_1692)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (415 amino acids)

>GFF4035 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VCVLGAGIVGLAAAWQLWREGFEVTVVDRAQAGTGASAANGAQLSYAYVQPLADPGIWRQ
LPKLLFAADSPLKLRLQPDPHQWAWGLQFLAACRAGVSRRTTASLLALAAESRAGFERLM
TEERIACDFSATGKLVLCADEASFAAARRQMALQRELGSPPQHALSPTECVAVEPALAGY
APRFAGAIHTPSECAADCHQVCLELLRLLLARGVRFAFDTPVTRLTRAGDRVVAAETPRG
PIEADAFVVALGTASHPLARTLGLRVPVYPLKGYSLTLDVSEAPGAAPRVNVTDAARKVV
FARLGSRLRVAGMAELVGENTHIPADRIASLAASAQALFPDARCGDDLRPWAGLRPATPT
GLPVLGRLRGGPDNLWFNTGHGALGFTLAFGSAQRLAAQLQLSLRSPATPAACSA