Protein Info for GFF4029 in Variovorax sp. SCN45

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 976 transmembrane" amino acids 355 to 374 (20 residues), see Phobius details amino acids 380 to 399 (20 residues), see Phobius details amino acids 411 to 428 (18 residues), see Phobius details amino acids 434 to 452 (19 residues), see Phobius details amino acids 582 to 604 (23 residues), see Phobius details amino acids 615 to 641 (27 residues), see Phobius details amino acids 915 to 937 (23 residues), see Phobius details amino acids 943 to 964 (22 residues), see Phobius details PF00403: HMA" amino acids 102 to 159 (58 residues), 36.6 bits, see alignment 7.2e-13 amino acids 196 to 240 (45 residues), 25 bits, see alignment 3.1e-09 amino acids 271 to 324 (54 residues), 26.8 bits, see alignment 8.5e-10 TIGR01511: copper-translocating P-type ATPase" amino acids 397 to 964 (568 residues), 403.3 bits, see alignment E=4.1e-124 TIGR01525: heavy metal translocating P-type ATPase" amino acids 413 to 964 (552 residues), 588.1 bits, see alignment E=4.3e-180 TIGR01494: HAD ATPase, P-type, family IC" amino acids 436 to 946 (511 residues), 270.9 bits, see alignment E=3.4e-84 PF00122: E1-E2_ATPase" amino acids 464 to 645 (182 residues), 185.7 bits, see alignment E=8.9e-59 PF00702: Hydrolase" amino acids 662 to 873 (212 residues), 124.4 bits, see alignment E=1.2e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 85% identity to dac:Daci_0479)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (976 amino acids)

>GFF4029 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Variovorax sp. SCN45)
MNAMTQTSCGSSCGCSTGDVKTPTAEPVVTGFAIANMDCPSEEAQIRKRLGQLDGIQGMT
FELAGRRLEVTHASAGQNAILRALHDIGMQAVVDTKGPRQVVYFIEQMDCPNEERQLRSV
LEPLAGVRAVEFDLKAHTLTVSHTLSDTTSIARTIEGLGMKPVAKTGGDAPSPASIAAAA
PLSSVAAPAIGGATRFFISNMDCPTEEATIRKRLGTIDGIEQLDFDLMNRRLDIQHHLPD
HAPILKALNDVGMKASVEQVGGAESQGRAVYLIEKMDCPTEEGLLRKALEGMPGVNALSF
NLMGRTLTVSHELADLAPVTAAIERLGMAPVLQSASEPTPSAPREFGSGISRGQWLRMAI
SGVLALGAEAMVFAGTPEASWPVILASLAAIGLGGIETLKKGWIALKTRSLNMNLLMTVA
VIGAALIGQWPEAAVVIWLFGIAEMIEALSLDRARNAIRKLMDLAPESALVRQPDGQWIE
VKADAVPVGGVVRVRPGERIALDGEVVAGQSSVNQAPITGESMPVEKAVGATVFAGTINE
RGTLEFRVTSRTGETTLDRIARSVQEAQGQRAPTQRFVDRFASIYTPAVFAVALAVAVIP
PLAFGQPWFEWVYKALVMLVIACPCALVISTPVTVVSGLAAAARRGILVKGGLYLEQGRH
LKSVALDKTGTLTHGRPALTDVIAQGTLTKGEALRLAASIDVLSEHPVATAIVAGHGDGA
LASVERFEAIPGRGVKGDVDGRTYYVGNHRLIEELGICSPELEAQLDALELQAKTAVVLA
TDREVLAVLGVADTVRETSRQAIEDLKSLGIEPVMLTGDNKKTAQAVATQVGITSAKGEL
LPQDKLQAIEELLTRGPVGMVGDGVNDAPALARSSIGFAMGAAGTDTAIETADVALMQDD
LRKLPEFVRLSQRVGGILTANIVFALGTKAIFMVLAFTGHASLWLAILADMGASLAVVFN
GLRLLRAPTAPQVDKR