Protein Info for PS417_20580 in Pseudomonas simiae WCS417

Annotation: isocitrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 571 PF20423: AceK_regulatory" amino acids 9 to 308 (300 residues), 351.4 bits, see alignment E=4.1e-109 PF06315: AceK_kinase" amino acids 310 to 564 (255 residues), 399.5 bits, see alignment E=6.9e-124

Best Hits

Swiss-Prot: 90% identical to ACEK_PSEPF: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 96% identity to pfs:PFLU4546)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U7A0 at UniProt or InterPro

Protein Sequence (571 amino acids)

>PS417_20580 isocitrate dehydrogenase (Pseudomonas simiae WCS417)
MSQSALAVAQMILDGFDDYREHFRQITDGARERFERAQWQQGQAASAARINLYEEKVSEV
TTRLREHFDDGTLLDIDTWPVVKSAYIGLIDLRFDDELSETWYNSIFCGLFSHDLISDGC
MFIHTTRPSLRRARAAQTRTYTPAGNIPQMLAQIFADYRFSEPYADLASDLHRLETQLRE
NLPDWVCKDPDLKVELFSSVLYRNKGAYLVGRIYTRDEQWPLVIPLLHREGQGIQIDALI
TDEAEVSIIFSFTRSYFMVDVPVPAEFVGFLKRILPGKHIAELYTSIGFYKHGKSEFYRA
LINHLATTDDQFIMAPGVRGMVMSVFTLPGFNTVFKIIKDRFSPSKNVNRATVIEKYRLV
KSVDRVGRMADTQEFADFRFPLSKFEPECLAELLEVAAGTVEVEGDTVLIRHCWTERRMT
PLNLYLDNANPAQVREALEDYGLAIKQLAAANIFPGDMLLKNFGVTRHGRVVFYDYDEIC
FLTEANFRHIPAPRTPEDEMASEPWYSIGPLDVFPEEFPPFLFADAGQRKLFDELHGELY
NADYWKGLQEAIRAGKVIDVFPYRRKDRDNE