Protein Info for PS417_20515 in Pseudomonas simiae WCS417

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 199 PF02525: Flavodoxin_2" amino acids 3 to 193 (191 residues), 182.3 bits, see alignment E=9.4e-58 PF03358: FMN_red" amino acids 3 to 169 (167 residues), 50.7 bits, see alignment E=1.6e-17

Best Hits

Swiss-Prot: 86% identical to AZOR2_PSEPF: FMN-dependent NADH-azoreductase 2 (azoR2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 98% identity to pfs:PFLU4529)

MetaCyc: 48% identical to FMN dependent NADH:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
RXN0-5375 [EC: 1.7.1.17]; 1.6.5.- [EC: 1.7.1.17]

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.- or 1.7.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UPV4 at UniProt or InterPro

Protein Sequence (199 amino acids)

>PS417_20515 FMN-dependent NADH-azoreductase (Pseudomonas simiae WCS417)
MSNVLIIESSARQQDSISRQLTQQFISQWQAAHPADQITVRDVALNPVPHLDANLLGGWM
KPADQRNDSEQASLDRSDELTRELLAADVLVMAAPMYNFAIPSTLKAWLDHVLRAGVTFK
YTATGPQGLLTGKRAIVLTARGGIHTGASSDHQEPYLRQVMAFIGIHDVTFIHAEGVNLS
GDFQEKGINHAKALLAQVA