Protein Info for GFF3994 in Xanthobacter sp. DMC5

Annotation: ATP-dependent RNA helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 835 TIGR01970: ATP-dependent helicase HrpB" amino acids 6 to 834 (829 residues), 990.6 bits, see alignment E=3.6e-302 PF00270: DEAD" amino acids 21 to 165 (145 residues), 32.6 bits, see alignment E=1.3e-11 PF00271: Helicase_C" amino acids 209 to 334 (126 residues), 42.9 bits, see alignment E=1e-14 PF04408: HA2_N" amino acids 399 to 424 (26 residues), 31.5 bits, see alignment (E = 2.8e-11) PF08482: HrpB_C" amino acids 689 to 821 (133 residues), 174 bits, see alignment E=3.7e-55

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 81% identity to xau:Xaut_2129)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (835 amino acids)

>GFF3994 ATP-dependent RNA helicase HrpB (Xanthobacter sp. DMC5)
MFDTKLPIDAVLGDIRSTLASTSCAVLVAPPGAGKTTRVPLALLDEPWAAGGKILVLEPR
RLAARAAARRMAQTLGEAVGGTVGFRTRLASEVSRRTRIEVVTEGIFTRMVLDDPELSGI
AAVLFDEFHERSLDADLGLALALDSQRGLRDDLRLLAMSATLDGARVASLMGLGAPAPVI
ESEGRAFPVETAYLGRDPRTPIDRQMTDAIRRALEREDGSILAFLPGAAEIRRTETLLLD
AVRDPKVDVVALFGALDAAEQDRAVLPAPEGRRKVVLATSIAETSLTIEGVRVVVDSGLA
RVPRFEPDVGLTRLETVRVSRAAADQRRGRAGRTEPGVCYRLWSEGETASLPAFATPEIL
AADLTGLVLDLARVGVRDPAALPFLDPPPAPAVTEAKALLRLLGALDADEAVTPLGRLMA
RLPLPPRLAHMVAIGAARGVGRLGAEIAALLVERGLGGDSVDLLARLDGFRRDRSRRAED
SRRLAGRWAEMAARELAADGRASDTAPPPSPSALLALAFPDRVAKAREGRAGETQMRFLM
TNGRGAVLEGTSPLARAPFLAVAEASGRAEGARITLAAALSTDEVEALFADDITSGLEVA
FDPAAMAVRAREVRRLKALVLAARPQPVPADAETARALARGIADADIGRLPFSAASRQWR
ERVMFLRAAEGEPWPDLSDAALAASVETWLAPFLSDRTSLADISGADVGNALHALLPYEL
TARLEAEAPTHFAAPTGSRLAIDYGAEGGPAVEVRVQELFGLTAHPAIAGGRVPLTLALL
SPAHRPIQVTKDLPQFWRGSWRDVRADMRGRYPKHPWPEDPAAAPPTTRAKPRGT