Protein Info for GFF3986 in Xanthobacter sp. DMC5

Annotation: Isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1009 TIGR00392: isoleucine--tRNA ligase" amino acids 29 to 904 (876 residues), 779.4 bits, see alignment E=2.5e-238 PF00133: tRNA-synt_1" amino acids 42 to 715 (674 residues), 431.7 bits, see alignment E=5.3e-133 PF09334: tRNA-synt_1g" amino acids 72 to 125 (54 residues), 41.9 bits, see alignment 1.2e-14 PF08264: Anticodon_1" amino acids 760 to 909 (150 residues), 78.7 bits, see alignment E=8.9e-26

Best Hits

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 82% identity to azc:AZC_4239)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1009 amino acids)

>GFF3986 Isoleucine--tRNA ligase (Xanthobacter sp. DMC5)
MTEKSETKPSAEASAPDYSKTLFLPETAFPMRGGLPQKEPELLARWARIGLYERLREAGR
GRDRFVLHDGPPYANGNIHIGHALNKILKDVVTRSQQMLGYDSNYVPGWDCHGLPIEWKI
EEENYRKKGKQKPDFSDAAAMVAFRQECRAYAEEWVGKQREEFKRLGVEGDWDHPYTTMA
FMAEAQIAREIMKFAENGLLYRGSKPVMWSVVEKTALAEAEVEYQDFTSDTVWVKFPVVS
EGDLKGATVVIWTTTPWTMPGNRAVTYAPKIAYGVYEVTDAPADNWAKTGDCLILADKLA
DDVFKAARVTAYERRGDVPADVLGSLILAHPLADLGLGGYTFKVPLLEAEHVTDDAGTGF
VHTAPGHGREDFEAWVNHRRWLEERGINPAIPYTVDEDSFYTPQAPGFEGKRVITEKGEK
GDANGAVIDALIKAGNLLARGRVKHQYPHSWRSKKPVIFRNTPQWFIAMDQDIRAADGKA
APRPATLAGNAPDTLRARALAGIKTVEWVPAAGENRITGMIENRPDWVVSRQRAWGVPIA
VFVKDKGDGTVEILKDAAVNARIAEAFAAEGADAWFKEGASARFLGERASEGWQKVDDVL
DVWFDSGSTHTFTLEVRPDLKAERPPEGRDKVMYLEGSDQHRGWFHSSLLESCGTRGRPP
YDVVLTHGFVLDEDGRKMSKSLGNVTSPQDVIKQSGADILRLWVCASDYADDLRIGPEIL
KTTADTYRKLRNTIRWLLGSLHHYRPEEKVAVADMPSLERFILHRLAELDGEIRAAYRAF
DYKKVHAALAQFMNIELSAFYFDIRKDALYCDPISSVTRRACLTVLDEVFNRLITWLAPI
LAFTCEEAYIARTGDEEGSVHLLAFPDTPAAWRDEALAEQWRKVRLIRRVVLGALEVERA
AKRMGSSLEAAPEVYVSDDALAEALSGLDLAEIAITSDAKLIRGEGPADAFRLADVPGVA
VVPVRAEGTKCARSWKVSPQVGTDLEFPDVTPRDAAALREFYAARAAAE