Protein Info for GFF397 in Variovorax sp. SCN45

Annotation: NAD(P)H-hydrate epimerase (EC 5.1.99.6) / ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 TIGR00197: YjeF family N-terminal domain" amino acids 24 to 212 (189 residues), 105.4 bits, see alignment E=3.1e-34 PF03853: YjeF_N" amino acids 32 to 193 (162 residues), 126.2 bits, see alignment E=2e-40 TIGR00196: YjeF family C-terminal domain" amino acids 231 to 473 (243 residues), 153.9 bits, see alignment E=5.4e-49 PF01256: Carb_kinase" amino acids 245 to 477 (233 residues), 146 bits, see alignment E=2e-46 PF08543: Phos_pyr_kin" amino acids 330 to 457 (128 residues), 27.3 bits, see alignment E=3.4e-10

Best Hits

KEGG orthology group: None (inferred from 81% identity to vpe:Varpa_3331)

Predicted SEED Role

"NAD(P)HX epimerase / NAD(P)HX dehydratase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (486 amino acids)

>GFF397 NAD(P)H-hydrate epimerase (EC 5.1.99.6) / ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136) (Variovorax sp. SCN45)
MQRITPATAADLFDIAATRRIEQAAAAALPAHALMQRAGLAVARLAMAIAPHARTIWVAC
GPGNNGGDGFEAAAQLRHRGFFPVVTFAGDESRLPPDAKTSLQRARDAGVTFAETPPTTF
ELAIDALLGIGSTRPPEGTMAEWLQLMHGSTQPVLSVDVPSGLNADTGVLPKDAASAITS
ARHCITFLTLKPGLFTAQGRDAAGTTWFDDLGCGHITDRPAARLTGTPKAQPRAHASHKG
SYGDVAVIGGASGMAGAALLAGSAALHAGAGRVFVGLLDPDAAVVDTSQTELMLRDARSL
DLSGMTVVCGCGGGEAVHELLPRVVTTAAALVLDADALNAIAADSALQAQLASRGKDGLP
TVITPHPLEAARLLGCTAADIQADRLASARQLASRFGVVAVLKGSGTVISDADGTPPVIN
LTGNARLATAGTGDVLAGMIGAALAAGRPVLEAASEAVWQHGHIADTWPADATPLTAGTL
ARRAPV