Protein Info for GFF3950 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 transmembrane" amino acids 134 to 153 (20 residues), see Phobius details amino acids 159 to 176 (18 residues), see Phobius details amino acids 188 to 205 (18 residues), see Phobius details amino acids 210 to 225 (16 residues), see Phobius details amino acids 360 to 380 (21 residues), see Phobius details amino acids 391 to 416 (26 residues), see Phobius details amino acids 693 to 713 (21 residues), see Phobius details amino acids 719 to 741 (23 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 172 to 740 (569 residues), 422.2 bits, see alignment E=7.3e-130 TIGR01525: heavy metal translocating P-type ATPase" amino acids 190 to 740 (551 residues), 602.9 bits, see alignment E=1.5e-184 TIGR01494: HAD ATPase, P-type, family IC" amino acids 212 to 721 (510 residues), 283.2 bits, see alignment E=6.4e-88 PF00122: E1-E2_ATPase" amino acids 241 to 341 (101 residues), 100.9 bits, see alignment E=4.9e-33 PF00702: Hydrolase" amino acids 439 to 651 (213 residues), 136.5 bits, see alignment E=2.2e-43

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 62% identity to avn:Avin_18520)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>GFF3950 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MHKENPTSLPAASTVLQVPDMCCAAEFNLVEKELRRVDGVHDVTPDFIRRSVRVAHQAVP
EPELIAAAARSGFEVRPGRALPQGKGLGSIAVRTVNAAPAAAAHADHEHDPGHKHEHEHE
EAPGHTHANSTPSWKLIVGGVLALAAELAALAYGDSSASAVALALGAIVLAGLETYRKGL
VALRRFNLNINALMTVAVTGAAILGQWPEAAMVIVLFAIAEMIEEKSLDRARRAIEGLMA
MAPDMATVQQGTTWQAVPAATVAVGAVVRVRPGERVALDGTVTAGSSAVDQAAITGESVP
VDKQVGDTLFAGTINQNGELQYTVSARADDSTLARIIRAVQEAQASRAPTQRFVDTFARY
YTPAVFAVAVAIAVLPPLLLTGADWLTWVYRALVLLVIACPCALVLSTPISVVSGLTAAA
RRGILVKGGLHLEQGHRLKVLALDKTGTLTQGRPVLTDVQALQGSEDEVLALAIALSSRS
DHPVSRAIAARGKGALQEVSDFQALQGRGVEGRIANERYRLGNHRLVEESAACSPDLEAR
LDALEAQGKTAVVLVRGDVPIGIFAIADQVRAESVEAVAQLKQLGIRPVMLTGDNRHTAE
AIARQVGIEDVRSELMPQDKLQAIEALSADGTVVGMVGDGINDAPALAKAHIGFAMGAAG
TDTAIETADVALMDDDPRKLAEFVDLSRATRAVLWQNITLALGIKVVFLALAVVGQATLW
MAVFADMGGSLLVVFNGLRLLRHRRR