Protein Info for GFF3946 in Sphingobium sp. HT1-2

Annotation: Peptidase, M16 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 965 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00675: Peptidase_M16" amino acids 86 to 210 (125 residues), 69.5 bits, see alignment E=3e-23 PF05193: Peptidase_M16_C" amino acids 236 to 416 (181 residues), 62.3 bits, see alignment E=5.8e-21 amino acids 711 to 891 (181 residues), 57.6 bits, see alignment E=1.6e-19

Best Hits

KEGG orthology group: K07263, zinc protease [EC: 3.4.24.-] (inferred from 85% identity to sjp:SJA_C1-20470)

Predicted SEED Role

"peptidase M16-like protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (965 amino acids)

>GFF3946 Peptidase, M16 family (Sphingobium sp. HT1-2)
MRLTSRRSAASLTILALLLSGSPLPLAARTDPSVAGAKAAPGGSQADIRPWLYENSDVPI
DTAWRFGTLSNGLRYAIRRNGVPPGQVSIRLRIDAGSLMERPDEQGYAHFMEHLSMRGSR
HVPDGESKRIWQRLGVTFGSDSNAATTPTGTTYALDLPQANQASLGESLKILAGMMTDPN
IVDSAVDAERAVVLAERRESDGPQMRISDASRQHFFAGQPLADHAPIGTVATLNAVTAAK
AEAFHQRWYRPENTVIAIAGDIDPALAEQLLKDNFASWQDSGKGAEQPDFGEPDAKAPAT
RVVVEPGSPTGLTMAWLRPWKPHADTIVYNQDKLTDMLALQIVSRRLEQAARAGGSFLAA
GVDQQDISRSADGTFITITPTGENWEKALADVRAIIEDAKAAPPTQQEIDREYAQLDTAL
AIQVENADTEAGAKQASDLVSAVDIRETTVSPQAALDIFRSGKPAMTPQKILACTRRLFS
AGVFRAMLITGKVEAGQDQKLAAALAAPVKAATNARLADKAVTMDDLPKFGAPATVVSRT
PVGLQGMESITFSNGVKLTLFANDAETEKVRINVRFGHGQQAFSPNKPVASWAGDYALVA
SGIGKLGQRELDDLTNGRRMGMQFSTDDDAFELQAVTRPADYKDQLRLFAAKLYQPGWDP
APIARVKTGASVAYDAMSRAPDGVLGRDLNWLLHDKDVRFRTPSKAEINALTPESFRQTW
EPILASGPIEIQIFGQVKAEEAIEAVAATFGAMPARPDVPVPAANKVQRFPAHVEQPVIL
RHDGDKEQAAAVMAWPTSGGFALTKEARQLEILTQIFNDRLFDKLRSTEGAAYSPSVQNS
WPFSYDSGGYILVTSQVRPDRVNYFYSVVKETAADLAKTPISADELQRAVAPMRQLLMRA
STGNAFWMGQMEGSTHDPRYVQAMQTMSQDLLNVTPADIQALATKYLVPGKSWSAVVLPQ
GVAAK