Protein Info for GFF3943 in Variovorax sp. SCN45

Annotation: Poly(A) polymerase (EC 2.7.7.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 TIGR01942: poly(A) polymerase" amino acids 30 to 470 (441 residues), 447.7 bits, see alignment E=2e-138 PF01743: PolyA_pol" amino acids 59 to 206 (148 residues), 114.3 bits, see alignment E=7.3e-37 PF12627: PolyA_pol_RNAbd" amino acids 238 to 298 (61 residues), 56.4 bits, see alignment E=3e-19 PF12626: PolyA_pol_arg_C" amino acids 358 to 468 (111 residues), 123.7 bits, see alignment E=6.2e-40

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 92% identity to vap:Vapar_1467)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (543 amino acids)

>GFF3943 Poly(A) polymerase (EC 2.7.7.19) (Variovorax sp. SCN45)
MIKKFIDKLLGKTAGGAQGKSRFGKRQEVPAEVHKIDPALVDERAKNVVTTLQQAGFEAY
VVGGAVRDLLLGLRPKDFDVATNATPEQVKSLFRRAFIIGRRFRIVHVVYGRGREHEVIE
VSTFRAYMDNTAAEQVSGNERTSKGELASMKHAVDASGRVLRDNVWGPQEEDAARRDFTV
NAMYYDPANQVVVDYHNGIKDAQKLTLRMIGDPATRYREDPVRIIRAIRFSAKLAALGFK
MEAKTAAPLVESSKLLADVPQSRLFDEMLKLLQTGHAIATVEQLNKLGLSKGIYPLLDVV
VERADQPFVKAALQDTDRRVGEGKPVAPSFLLACVLWADVRDGWAQRQEGQRGQRPQAPF
PALQDAIDDVFNARIGDVSGRGKLAADMREIWMMQPRFDKRTGSTPYSLVEQARFRAAFD
FMRLRADVGEVSEAIAEWWQEFSIADDVRRQDLLEQVREEQKVRQRVRVRDTAAPAKLRN
EPAPRQNPPQNRGQDAEPDDEAEVEAGAVPPDGEAAAPRKRRRRRKPRPAGGGGEGGGSA
AGE