Protein Info for GFF3942 in Xanthobacter sp. DMC5

Annotation: Primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 PF17764: PriA_3primeBD" amino acids 7 to 103 (97 residues), 73.1 bits, see alignment E=4.2e-24 PF04851: ResIII" amino acids 192 to 355 (164 residues), 40.1 bits, see alignment E=1.1e-13 TIGR00595: primosomal protein N'" amino acids 217 to 722 (506 residues), 591.6 bits, see alignment E=6.2e-182 PF00270: DEAD" amino acids 217 to 358 (142 residues), 57 bits, see alignment E=6.5e-19 PF18319: Zn_ribbon_PriA" amino acids 443 to 469 (27 residues), 36.9 bits, see alignment (E = 8.3e-13) PF18074: PriA_C" amino acids 631 to 722 (92 residues), 63.6 bits, see alignment E=7.6e-21

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 88% identity to xau:Xaut_2053)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (726 amino acids)

>GFF3942 Primosomal protein N' (Xanthobacter sp. DMC5)
MSPRTVDVLLPLGLDAAYSYLLPEGMEVSPGDLVHVPLGTKSVIGVVWPRTQAAGGKAVE
AGKLKAVKAKLDYVPLPPDLIKLIDWMADYTLAPRGMVLRMALRHGESPGAARERVGVRL
TGTAPARMTAARARLLDTLADGFVRGKTEAAREAGVSPSVVDGLVDDGTLEAVVLPPEPA
AYPPDPDHAVPELSQPQAEAAAAMVAAVKAKTFAVHLLDGVTGSGKTETYFEAVAEAIRA
GRQALILLPEIALTQAFLDRFTERFGVKPAEWHSGVSTRRRAKVLNEVASGAVKVVAGAR
SALFLPFRDLGLIIVDEEHDPAYKQEDGVCYHARDMAVVRARLASAPIVLASATPSIETE
VNARRGRYRRLALPVRFGGAKVPGLSPIDLRKEGPPRGRWIAPRLEAEINTTLEAGEQAL
LFLNRRGYAPLTLCRSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEKCPACDAP
DSLVPVGPGVERLQEEVQTLFPNARALVLSSDLLGGVERMRAELDAVAKGDVDVVVGTQL
VAKGHNFPGLALVGVVDADVGLGHGDPRAAERTFQLVHQVAGRAGRGKAEGRGFIQTHMP
EHPVMRALVTGDRDAFYDVEIASREEAHLPPFGRMAALIVSASEAHVAEGHARRLATSAP
IVPDVRILGPAEAPLAILRGRHRWRLLVRSPRNFDLPAYLREWRAVAPKVTGNIKVAIDV
DPQSFL