Protein Info for PS417_20150 in Pseudomonas simiae WCS417

Annotation: (p)ppGpp synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF13328: HD_4" amino acids 40 to 202 (163 residues), 153.7 bits, see alignment E=8.7e-49 TIGR00691: RelA/SpoT family protein" amino acids 69 to 742 (674 residues), 618.7 bits, see alignment E=6.4e-190 PF04607: RelA_SpoT" amino acids 262 to 370 (109 residues), 147.3 bits, see alignment E=5.3e-47 PF02824: TGS" amino acids 416 to 475 (60 residues), 81.5 bits, see alignment 9.3e-27 PF19296: RelA_AH_RIS" amino acids 494 to 638 (145 residues), 32.6 bits, see alignment E=2.2e-11 PF13291: ACT_4" amino acids 667 to 743 (77 residues), 65.8 bits, see alignment E=1.1e-21

Best Hits

Swiss-Prot: 53% identical to RELA_ECOLI: GTP pyrophosphokinase (relA) from Escherichia coli (strain K12)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to pfs:PFLU4505)

MetaCyc: 53% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UPN5 at UniProt or InterPro

Protein Sequence (747 amino acids)

>PS417_20150 (p)ppGpp synthetase (Pseudomonas simiae WCS417)
MVQVRAHQPINTDGSINLEAWLDHAISVDPALDREALKAACEFARESEQQDNASKNLWAE
GTSSFRTGLEIAEILADLKLDQDSLIAAVLYRGVREGHIQLATVGQRFGSVVAKLIDGVL
RMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKTADDE
KRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIATLLHERRLDRERFI
TDVMNQLRSELQATGVEADISGRAKHIYSIWRKMQRKGLAFSQIYDVRAVRVLVPEMRDC
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAEL
GVCAHWRYKGTDVKAGSNQYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYIFT
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYITTSRARAKIVHWFKLQARDQNVAAGKTLLERELGRLGLPQVDFDKLA
EKANMKIAEDMFAALGAGDLRLAQLVNLAQQLVEPERGSEQLELIPRKATGYKPGKRGDI
QIQGVGNLMTQIAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLGGREPERIIQVSW
GPVPVLTYPVDIIIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP