Protein Info for GFF3935 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Homoserine dehydrogenase (EC 1.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03447: NAD_binding_3
  • amino acids 13 to 132 (120 residues), 69.3 bits, E=9.8e-23
• PF00742: Homoserine_dh
  • amino acids 140 to 318 (179 residues), 216.5 bits, E=4.9e-68
• PF01842: ACT
  • amino acids 357 to 424 (68 residues), 39.7 bits, E=6.4e-14

Best Hits

Swiss-Prot: 63% identical to DHOM_PSEAE: Homoserine dehydrogenase (hom) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00003, homoserine dehydrogenase [EC: 1.1.1.3] (inferred from 89% identity to vap:Vapar_1831)

Predicted SEED Role

"Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Methionine Biosynthesis or Threonine and Homoserine Biosynthesis (EC 1.1.1.3)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-homoserine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3

Use Curated BLAST to search for 1.1.1.3

Protein Sequence (443 amino acids)

>GFF3935 Homoserine dehydrogenase (EC 1.1.1.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTDMKPIQVGLLGIGTVGSGTFNVLQRNQEEIRRRAGRGIEITMVADLDVARAQAIVGPD
VKVVNDARAVIANPEIDIVIELIGGYGIAKALVMEAIAAGKHVVTANKALLAVHGTEIFA
AASAKGVMVAFEAAVAGGIPIIKALREGLTANSIQWIAGIINGTTNFILSEMRDKGLDFA
VVLQEAQRLGYAEADPTFDIEGVDAAHKVTLMSAIAFGIPVQFDKAYVEGITQLGATDIK
YAEQLGYRIKLLGITKRTAKGIELRVHPSLVPAKRLIANVEGAMNAVVVNGDAVGTTLYY
GKGAGSEPTASAVIADLVDITRLHTADPHHRVPHLAFQPDALSDVAVLPMSEVVTSYYLR
LRVADQAGVLAKVTGLLAGAGISIDAVLQREADEVSGEGGTSTDLIILTHDTREGTMNDA
LAQLQTLPTVLAPIVRIRKEELN