Protein Info for PS417_20125 in Pseudomonas simiae WCS417

Annotation: phosphoribosylglycinamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00551: Formyl_trans_N
  • amino acids 7 to 185 (179 residues), 218.5 bits, E=3e-69
• TIGR00639: phosphoribosylglycinamide formyltransferase
  • amino acids 7 to 193 (187 residues), 253.3 bits, E=6.1e-80

Best Hits

Swiss-Prot: 59% identical to PUR3_ECOLI: Phosphoribosylglycinamide formyltransferase (purN) from Escherichia coli (strain K12)

KEGG orthology group: K11175, phosphoribosylglycinamide formyltransferase 1 [EC: 2.1.2.2] (inferred from 98% identity to pfs:PFLU4500)

MetaCyc: 59% identical to phosphoribosylglycinamide formyltransferase 1 (Escherichia coli K-12 substr. MG1655)
Phosphoribosylglycinamide formyltransferase. [EC: 2.1.2.2]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.2)

MetaCyc Pathways

• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)

KEGG Metabolic Maps

• One carbon pool by folate
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2

Use Curated BLAST to search for 2.1.2.2

Sequence Analysis Tools

See U1TTA3 at UniProt or InterPro

Protein Sequence (216 amino acids)

>PS417_20125 phosphoribosylglycinamide formyltransferase (Pseudomonas simiae WCS417)
MPQTCDVVVLLSGTGSNLQALIDSTRTGDSPVRIAAVISNRSDAYGLQRARDAGIDTRSL
DHKAFEGREAFDSALIELIDAFNPKLVVLAGFMRILSADFVRHYEGRLLNIHPSLLPKYK
GMHTHQRALDAGDSEHGCSVHFVTEELDGGPLVVQAVVPVESDDSAQTLAQRVHTQEHRI
YPLAVRWFAEGRLILGDQGALLDGQLLAASGHLIRT