Protein Info for GFF393 in Methylophilus sp. DMC18

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details amino acids 61 to 81 (21 residues), see Phobius details amino acids 88 to 109 (22 residues), see Phobius details amino acids 134 to 152 (19 residues), see Phobius details amino acids 164 to 185 (22 residues), see Phobius details amino acids 191 to 208 (18 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details PF00563: EAL" amino acids 296 to 531 (236 residues), 265.6 bits, see alignment E=1.8e-83

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (550 amino acids)

>GFF393 hypothetical protein (Methylophilus sp. DMC18)
MSQNGIGELAAPLNENTTQRQFMRHRLVRKVQDNTHFLAMTSMVCGLLGFPHYLLDVDNN
IWLAQLLTYLTFILVSYNLLLENGRNNLAMLTMSLVTISLFVVQLSPYAADFNDTASILR
ASGQTGLHAPSNSWEFIAGGVLACLIAVSYLFQRHHYVAASQGMLCIAVMIPLIPLLGLL
CGIANPYGVMSPLTTLSGMLCAFGLLAKQASHPPMSYLLLMDDTGKQIRSSILLLSICAI
LLARAGVMFGDNMRGLPLVVLVSLIAIIYTLSVTYYHQGQVSEKVLPATDMDFASQVETA
LDNQQFFMMYQPQVDFKTGKLKGVEALVRWRHPEKGIISPEKFIRVAELTGLIVPLGKWI
MQTACAQAAQWKDHPVLGKIEVSVNVSALQLKSGTLVEEIMQILQDTGLAPERLVIELTE
SSFVQDDGENARLMHQLKEAGIKLAIDDFGTGYSCLSYLRDIPGDYLKVDRSFVMELPGH
NKAEAVIQAIVSLGKSLDYRIIAEGVETQAQADFLKSIGCDIVQGFLYAKPLEADALPQW
VSVAQPETLT