Protein Info for GFF3891 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: L-lactate dehydrogenase (EC 1.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 21 to 362 (342 residues), 411.1 bits, E=1.9e-127

Best Hits

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 72% identity to pna:Pnap_2560)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• methylglyoxal degradation V (2/3 steps found)
• photorespiration I (4/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3, 1.1.3.15

Use Curated BLAST to search for 1.1.2.3 or 1.1.3.15

Protein Sequence (370 amino acids)

>GFF3891 L-lactate dehydrogenase (EC 1.1.2.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTPHPALPPDVATLGDHERHARRALDDATWAYLFGAAADELTFARNIDAWRALELLPRVL
QPLAGGHTRVELLGRTLAHPILVAPMAYQRLAHPDGEHATALACAALGAGLVFSTQASAP
LDEIAAAFLPEPGRGPLWFQLYLQADRGFTADLVRRAEAAGFEALVLTVDAPVQGARDSE
RRSGFRLPANVSAVNLAGLRPPPPRALAAGQSPLFDDLLTHAPGWDDVAWLRSITRLPVL
LKGILHPGDARQAVAAGASGLIVSNHGGRTLDTAVTTASALPRIAQAVQGDLPLLVDGGI
RRGTDVLKAVGLGASAVLVGRPVLHGLATAGATGVAHVLRLLRDELEIAMALTGCRTLAE
ASTVLAPASR