Protein Info for GFF3888 in Variovorax sp. SCN45

Annotation: 3-isopropylmalate dehydrogenase (EC 1.1.1.85)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 2 to 350 (349 residues), 499.8 bits, E=1.9e-154
• PF00180: Iso_dh
  • amino acids 2 to 351 (350 residues), 459 bits, E=6.1e-142

Best Hits

Swiss-Prot: 88% identical to LEU3_RHOFT: 3-isopropylmalate dehydrogenase (leuB) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 98% identity to vpe:Varpa_1565)

MetaCyc: 57% identical to isopropylmalate dehydrogenase 3 (Arabidopsis thaliana col)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Protein Sequence (362 amino acids)

>GFF3888 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (Variovorax sp. SCN45)
MKIAVLPGDGIGTEIVAEAVKVLNVLDLKFEMESALVGGAAYEAHGHPLPESTLKLAKES
DAVLFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYEQLVDASSLKPELIA
GLDILIIRELTGDIYFGQPRGRRIATDGHFPGAEEAFDTMRYSRPEIERIARVAFEAARK
RSKRVTSVDKNNVLETSQLWKDVMLDIAKEYPDVELDHMLVDNAAMQLVKAPKKFDVVVT
GNMFGDILSDEAAMLTGSIGMLPSASLNSSNQGLYEPSHGSAPDIAGKGVANPLATILSA
AMMLRFSLNQAEAADRIESAVKDVLASGLRTGDIWSEGTKRVGTREMGDAVVAAITKKTI
TG