Protein Info for GFF3886 in Xanthobacter sp. DMC5

Annotation: Folylpolyglutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 29 to 433 (405 residues), 359.2 bits, E=1.9e-111
• PF08245: Mur_ligase_M
  • amino acids 52 to 272 (221 residues), 31.6 bits, E=1.7e-11
• PF02875: Mur_ligase_C
  • amino acids 309 to 425 (117 residues), 31 bits, E=4.3e-11

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 90% identity to xau:Xaut_1697)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (41/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (441 amino acids)

>GFF3886 Folylpolyglutamate synthase (Xanthobacter sp. DMC5)
MALPLPARATDEILSRLAALHPKKIDLSLARMERLLEALGHPERRLPKVIHVAGTNGKGS
TIAFMRGMLEAAGQSVHVYTSPHLVRFNERIRLGAPGGGQMVSDAALADALDRAEAANAG
APITLFEITTAAGFLLFAEHPADVLLLEVGLGGRLDATNVVDKPLASVITPVSIDHVDFL
GTSVEAIAGEKAGIIKPRVPVVVGAQSPDALAVIERAAAKKLAPLFVRGQQWNVQEESGR
LVYADEDGLLDLPRPRLVGPHQIDNAGLAVAALRAVKLPAAHDAGILRAEWPARLQRLAP
GPLSARAPAGVELWLDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDVTGFLQP
FAGLVREAIAVPVPGEHAGLKPEDVAAAAVGLGMKASVARDVGSALDGIAREAVVPPPRV
LIAGSLYLAGAVLAANGTALG