Protein Info for GFF3883 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: Colanic acid biosysnthesis protein WcaK
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to WCAK_ECOLI: Colanic acid biosynthesis protein WcaK (wcaK) from Escherichia coli (strain K12)
KEGG orthology group: None (inferred from 99% identity to sei:SPC_1620)MetaCyc: 68% identical to colanic acid biosynthesis pyruvyl transferase WcaK (Escherichia coli K-12 substr. MG1655)
2.5.1.-
Predicted SEED Role
"Colanic acid biosysnthesis protein WcaK" in subsystem Colanic acid biosynthesis
MetaCyc Pathways
- colanic acid (Escherichia coli K12) biosynthesis (9/11 steps found)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (426 amino acids)
>GFF3883 Colanic acid biosysnthesis protein WcaK (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLY QLSQKQQAAAGLNGRVKKVLRRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDA VIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGPFQNPDFNQLANYVFGRTSAL ILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMA KYVNDSENFHIAMDELNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAG IMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKVANAVTEERAKGFTMIKSVLD RIREAQ