Protein Info for GFF388 in Variovorax sp. SCN45

Annotation: Phosphatidate cytidylyltransferase (EC 2.7.7.41)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 18 to 20 (3 residues), see Phobius details amino acids 26 to 44 (19 residues), see Phobius details amino acids 53 to 73 (21 residues), see Phobius details amino acids 79 to 100 (22 residues), see Phobius details amino acids 111 to 129 (19 residues), see Phobius details amino acids 136 to 159 (24 residues), see Phobius details amino acids 180 to 198 (19 residues), see Phobius details amino acids 218 to 238 (21 residues), see Phobius details PF01148: CTP_transf_1" amino acids 3 to 279 (277 residues), 176.6 bits, see alignment E=4.6e-56

Best Hits

KEGG orthology group: K00981, phosphatidate cytidylyltransferase [EC: 2.7.7.41] (inferred from 92% identity to vpe:Varpa_3322)

Predicted SEED Role

"Phosphatidate cytidylyltransferase (EC 2.7.7.41)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.7.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.41

Use Curated BLAST to search for 2.7.7.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (281 amino acids)

>GFF388 Phosphatidate cytidylyltransferase (EC 2.7.7.41) (Variovorax sp. SCN45)
MLKQRILTAIVLLAILLPALFYRNYIPFACVMLVLIGAAAWEWGRLNGYGQRLSVFLGVE
TVLLCALSWWLGLLEQSLVLMWTLAGAAWVLGGAALLRVAVPGWPRIPRGLRLVGGLLAL
WVAWLAAVQARMVGINFLLSILVLVWVADVFAYFAGRAFGLKFTRNKLAPAISPGKSWEG
VWGGMIGVVVLAFAWVWADKAAGATVASLYTRLNERGWWLLLLGALFLAAMSVVGDLVES
LIKRSAGAKDSSKLLPGHGGVLDRVDALLPALPIAMMLASL