Protein Info for GFF3839 in Xanthobacter sp. DMC5

Annotation: Argininosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00838: argininosuccinate lyase
  • amino acids 5 to 450 (446 residues), 599.8 bits, E=1.9e-184
• PF00206: Lyase_1
  • amino acids 8 to 302 (295 residues), 281.6 bits, E=9.3e-88
• PF14698: ASL_C2
  • amino acids 365 to 433 (69 residues), 90.5 bits, E=7.6e-30

Best Hits

Swiss-Prot: 92% identical to ARLY_XANP2: Argininosuccinate lyase (argH) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 92% identity to xau:Xaut_1738)

MetaCyc: 44% identical to argininosuccinate lyase (Homo sapiens)
Argininosuccinate lyase. [EC: 4.3.2.1]

Predicted SEED Role

"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• urea cycle (3/5 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.1

Protein Sequence (465 amino acids)

>GFF3839 Argininosuccinate lyase (Xanthobacter sp. DMC5)
MSNKMWGGRFSTAPDAIMEEINASIGFDQRLYAQDIAGSKAHAAMLAAQGIVAAKDAQKI
TKGLDTILSEIEAGKFSFRRELEDIHMNVESRLAELIGAPAGRLHTARSRNDQVATDFRL
YVRDTVDALDAQLADLQRALAEKALAHAATVMPGFTHLQTAQPVTFGHHLMAYVEMMARD
RSRFADARARLNECPLGSAALAGTSFPIDREATAAALGFDRPTANSLDAVSDRDFVMETL
SAASICAIHLSRFAEEVVIWTSPSFALVKLSDAFTTGSSIMPQKRNPDAAELVRAKAGRI
IGALTGILVVMKGLPLAYAKDMQEDKEGAFDAFSALSLVIAATAGMVRDLSPDEKRMAAA
AGQGYSTATDLADWLVRALNIPFREAHHITGRIVALASDRGIALHKVSLEDMQAVDKRIT
GDVFSVLSVVRSVRSRTSYGGTAPQRVRAQARRWLKRLGAAPDAT