Protein Info for GFF3835 in Xanthobacter sp. DMC5

Annotation: Aerobic cobaltochelatase subunit CobT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 632 PF06213: CobT" amino acids 13 to 276 (264 residues), 326.7 bits, see alignment E=1.4e-101 TIGR01651: cobaltochelatase, CobT subunit" amino acids 17 to 616 (600 residues), 969.8 bits, see alignment E=3.1e-296 PF11775: CobT_C" amino acids 398 to 615 (218 residues), 391.1 bits, see alignment E=1.5e-121

Best Hits

Swiss-Prot: 62% identical to COBT_SINSX: Aerobic cobaltochelatase subunit CobT (cobT) from Sinorhizobium sp.

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 89% identity to xau:Xaut_1878)

MetaCyc: 62% identical to CobT (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (632 amino acids)

>GFF3835 Aerobic cobaltochelatase subunit CobT (Xanthobacter sp. DMC5)
MATNRTTGKAPKEAPSEPFKRAVTGCFKAIAGVSEFEVSFASERPALGPDRVRLPEPPRR
FTLQDAAIVRGHADSLALRRACHDPGVHRKMLPQGEVARGVFEAVEQARVEAIGGNRMPG
VKRNLAAMLQDRYHRANYADITERSEAPIADAVALMVRERLTGEAPPPDARRVVDLWRPF
IEERAAGDLARLIDSIENQKKFGEIARDLLASLDMGEESGLDTEQEPEENENEGGAEEQG
GENGESREDDSQQGATEMEAELSEEEMPDSAAQSEAAPPADVPDGTEMGDSEEPAEPWSP
RPPMTHDNRGPEYHAFTTKFDEETSAEDLCDPEELTRLRSYLDKQLAHLQGVVARLANRL
QRKLLAQQNRAWEFDLEEGVLDPARLPRVIMDPMAALSFKRERDTDFRDTVVTLLIDNSG
SMRGRPITVAATCADILARTLERCGVKVEVLGFTTRAWKGGQSREAWLAAGKPAAPGRLN
DLRHIVYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLARTEARRILMMIS
DGAPVDDSTLSVNAGNYLERHLRHIITEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAE
ELGGVMTEKLAELFEETPGAAGKGPARGGRLH