Protein Info for GFF3835 in Sphingobium sp. HT1-2

Annotation: Malyl-CoA lyase (EC 4.1.3.24)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 7 to 212 (206 residues), 126.8 bits, E=7.8e-41
• PF15617: C-C_Bond_Lyase
  • amino acids 161 to 270 (110 residues), 37.5 bits, E=1.7e-13

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 82% identity to sjp:SJA_C1-26890)

Predicted SEED Role

"Malyl-CoA lyase (EC 4.1.3.24)" in subsystem Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 4.1.3.24)

MetaCyc Pathways

• 3-hydroxypropanoate cycle (10/13 steps found)
• citrate degradation (2/2 steps found)
• methylaspartate cycle (13/19 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• glyoxylate assimilation (8/13 steps found)
• reductive TCA cycle II (6/12 steps found)
• ethylmalonyl-CoA pathway (5/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (4/14 steps found)

KEGG Metabolic Maps

• C5-Branched dibasic acid metabolism
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6

Use Curated BLAST to search for 4.1.3.24 or 4.1.3.34 or 4.1.3.6

Protein Sequence (271 amino acids)

>GFF3835 Malyl-CoA lyase (EC 4.1.3.24) (Sphingobium sp. HT1-2)
MQLRHARSLLFLPASNPRAVAKARTLACDMVILDLEDAVPDADKAAALDAAVEAVGEGFG
GRLMAVRINVEGMPSHGPEMVALKRTAADYVVLPKVENAKQVKDVYSVAQKPVIAMIESA
KGVLAAPAIAAGEGCAALFVGTNDLRKDLSIPPSAGRAGLAHVLQSVVLAARAAGIAVFD
GVFNRLDDEAGLEAECAEGHGFGFDGKTLIHPGQVPVANQAFGPDAQAVADARRLIEAAT
GGAERFEGRMIEAMHVDEARALVARAEAVGQ