Protein Info for GFF3830 in Xanthobacter sp. DMC5

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 TIGR01059: DNA gyrase, B subunit" amino acids 13 to 804 (792 residues), 976 bits, see alignment E=4.4e-298 PF02518: HATPase_c" amino acids 40 to 180 (141 residues), 68.2 bits, see alignment E=2.4e-22 PF00204: DNA_gyraseB" amino acids 232 to 401 (170 residues), 175.4 bits, see alignment E=2.4e-55 PF01751: Toprim" amino acids 430 to 542 (113 residues), 66.5 bits, see alignment E=5.8e-22 PF21249: GyrB_hook" amino acids 544 to 570 (27 residues), 32.1 bits, see alignment (E = 2e-11) PF00986: DNA_gyraseB_C" amino acids 737 to 798 (62 residues), 95.2 bits, see alignment 6.2e-31

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 86% identity to azc:AZC_1009)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (808 amino acids)

>GFF3830 DNA gyrase subunit B (Xanthobacter sp. DMC5)
MVEPVPAPSPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL
AGWAKEVTVTLEANGSVTVTDDGRGIPTDLHPEEGVSAAEVIMTQLHAGGKFNQNSYKVS
GGLHGVGVSVVNALSTTLDLTIWRDGKEHYMRFRHGDAEAPLKVVGTAPAGKRGTKVTFL
PSPETFTRIEFDYATLEHRLRELAFLNSGVLIVLTDARGPEPKREELHYEGGVEAFVKYL
DRNKTALIAKPVVIRAEKDGITVEAALSWNDGYHENVLCFTNNIPQRDRGTHFTGFAAAL
TRQVLGYANTSGISKKEKVDPTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVV
ENLVGEALANWFEENPVEAKNVIGKVVEAAAAREAARKARDLTRRKSPLDVASLPGKLAD
CQEKDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGT
LITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPEIVDAGHLFI
AQPPLYKVARGKSETYLKDEKALEDYLITTGLEEASLHLATGEVRTGADLAHVVDVSRQL
RSALSALHPRYNRAVAEQAALAGAFAPAALEDAATGSAAAAEVARRLDALADETERGWTG
ICDETGYVFSRTVRGVAEVARLDHAFIASPEARRLDTLARDLDGVFAAAATLKRRSDEQV
LFGAVDLFDAVTDSGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVRVKEVDAADD
LFSRLMGDTVEPRREFIQENALRASVDV