Protein Info for GFF3827 in Sphingobium sp. HT1-2

Annotation: Ferredoxin--NADP(+) reductase (EC 1.18.1.2) @ Flavodoxin--NADP(+) reductase (EC 1.19.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 56% identical to FENR_RHOCA: Flavodoxin/ferredoxin--NADP reductase (fpr) from Rhodobacter capsulatus

KEGG orthology group: K00528, ferredoxin--NADP+ reductase [EC: 1.18.1.2] (inferred from 89% identity to sjp:SJA_C1-10850)

Predicted SEED Role

"Flavodoxin reductases (ferredoxin-NADPH reductases) family 1" in subsystem Anaerobic respiratory reductases

MetaCyc Pathways

ethene biosynthesis V (engineered) (17/25 steps found)
superpathway of photosynthetic hydrogen production (3/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (1/4 steps found)
photosynthesis light reactions (1/4 steps found)
oxygenic photosynthesis (10/17 steps found)

KEGG Metabolic Maps

Photosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.18.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (282 amino acids)

>GFF3827 Ferredoxin--NADP(+) reductase (EC 1.18.1.2) @ Flavodoxin--NADP(+) reductase (EC 1.19.1.1) (Sphingobium sp. HT1-2)
MAAPTKCKDKVLTDVTIEKPVLEPTGALSVETVLSVKHWNEHLFSFRITRPASFRFRSGE
FIMIGLKGDNGKPLLRAYSIASPAWDEEIEFLSIKVQDGPLTSKLQKIEPGDQIYLGRKP
TGTLVTDALLPGKRLFMLSTGTGLAPFLSLPRDPDVYEFYEQVVVVHSVRRVSDLAFREE
MEGKWAEDPLVSEQAAGQFHYVPTVTREEFPNNERIDKLVESGKLFEGIPGAAKFDPETD
RIMMCGSMEMIKQFAAYFEEQGFTEGSNAAPGQFVIERAFVG