Protein Info for GFF3825 in Variovorax sp. SCN45

Annotation: Autolysin sensor kinase (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 transmembrane" amino acids 15 to 35 (21 residues), see Phobius details amino acids 41 to 64 (24 residues), see Phobius details amino acids 79 to 100 (22 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details PF06580: His_kinase" amino acids 140 to 218 (79 residues), 84.2 bits, see alignment E=3.2e-28

Best Hits

KEGG orthology group: K08082, two-component system, LytT family, sensor histidine kinase AlgZ [EC: 2.7.13.3] (inferred from 93% identity to vpe:Varpa_1502)

Predicted SEED Role

"Autolysis histidine kinase LytS" in subsystem Murein hydrolase regulation and cell death

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (332 amino acids)

>GFF3825 Autolysin sensor kinase (EC 2.7.3.-) (Variovorax sp. SCN45)
LRGGPSLFDACRIGVVLRTVVFVEAVVAIATLFVVSTPTEWLVQAATVTGGALPATLLWL
VATCGLKKPLRRLPRQWQYGAGGALGAIAALYGCGLLRLTGVLSNAPWLASALTGALFAG
LVMAAMVLRARGQTPAATTARLEELQSRIRPHFLFNTLNSAIALVREEPAKAETMLEDLA
ELFRQALADPGESGTLADEIALAERYLAIEQVRFGDRLRIRWDLDAAASTARLPPLLLQP
LVENAIKHGVEPSPEGAKLRIRTERRGSMVVIEVVNSLPPLRWADEPLPRGHGIALANVR
DRLRLLHDMQMQFSAGMDQKNYRVRIAIPAES